雄性和雌性 Wistar 大鼠心肌梗死后肾素-血管紧张素系统和激肽释放酶-激肽系统表达的差异。
Differences in the expression of the renin angiotensin system and the kallikrein-kinin system during the course of myocardial infarction in male and female Wistar rats.
机构信息
Laboratorio de Farmacologia del Miocardio, Universidad Nacional Autonoma de Mexico, Mexico.
Laboratorio de Inmunobiología de Enfermedades Infecciosas, Universidad Nacional Autonoma de Mexico, Mexico.
出版信息
J Renin Angiotensin Aldosterone Syst. 2020 Apr-Jun;21(2):1470320319900038. doi: 10.1177/1470320319900038.
BACKGROUND
There is some evidence that components of the renin-angiotensin system and kallikrein-kinin system are not similarly regulated in both sexes. The aim of this work was to analyze the expression of angiotensin-converting enzyme, angiotensin-converting enzyme 2, angiotensin 1 receptor, angiotensin 2 receptor, beta-1 receptor, and beta-2 receptor during the evolution of myocardial infarction.
METHODS
Thirty-six male and 36 female Wistar rats were used. Myocardial infarction was induced. Six groups of both sexes were formed, (=6): (a) sham; (b) 48 h myocardial infarction; (c) one week myocardial infarction; (d) two weeks myocardial infarction; (e) three weeks myocardial infarction and (f) four weeks myocardial infarction. The expression was evaluated by real-time polymerase chain reaction on the penumbra of left ventricle.
RESULTS
The mRNA expression of most biomarkers was lower in females than in males. During acute infarction, an increase of all protein expression was found in female and at two weeks while in the male only biomarker changes occurred at three weeks. In addition, in male biomarkers mRNA expression decreased during chronic infarction while in females it did not.
CONCLUSIONS
The renin-angiotensin system and kallikrein-kinin system biomarkers expression occurs at earlier times in the female than in the male rat. In addition, during chronic myocardial infarction these biomarkers remained unchanged in females while in males they decreased.
背景
有证据表明肾素-血管紧张素系统和激肽释放酶-激肽系统的成分在两性中并非相似调节。本研究旨在分析血管紧张素转换酶、血管紧张素转换酶 2、血管紧张素 1 受体、血管紧张素 2 受体、β-1 受体和β-2 受体在心肌梗死演变过程中的表达。
方法
使用 36 只雄性和 36 只雌性 Wistar 大鼠。诱导心肌梗死。将雌雄各 6 组分为：（a）假手术；（b）心肌梗死后 48 小时；（c）心肌梗死后 1 周；（d）心肌梗死后 2 周；（e）心肌梗死后 3 周和（f）心肌梗死后 4 周。通过左心室半影实时聚合酶链反应评估表达。
结果
大多数生物标志物的 mRNA 表达在雌性中低于雄性。在急性梗死中，所有蛋白表达均增加，而在雌性中则在 2 周时增加，而在雄性中仅在 3 周时发生标志物变化。此外，在慢性梗死过程中雄性标志物的 mRNA 表达下降，而在雌性中则没有。
结论
肾素-血管紧张素系统和激肽释放酶-激肽系统生物标志物在雌性大鼠中的表达早于雄性。此外，在慢性心肌梗死后，这些标志物在雌性中保持不变，而在雄性中则减少。
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