Department of Biomedical Engineering, Research Center for Nano-Biomaterials and Regenerative Medicine, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan, China.
Shanxi Key Labratory of Materials Strength and Structrual Impact, Taiyuan University of Technology, Institute of Biomedical Engineering, Taiyuan, China.
Tissue Eng Part C Methods. 2020 Jul;26(7):352-363. doi: 10.1089/ten.TEC.2020.0073.
This study aimed at designing a novel electrospun scaffolding material that structurally and chemically resembles native extracellular matrix for bone tissue engineering. Calcium chloride-complexed polyamide66 (PA66/CaCl) and pure PA66 electrospun nanofibers were fabricated by the electrospinning method. Scanning electron microscopy, X-ray diffraction, and Fourier transform infrared (FTIR) spectroscopy were used to investigate the effect of the presence of ionized salt in the polymer solution on the mechanical properties and other properties of the electrospun scaffolds. The results show that addition of CaCl to PA66 solution can achieve an internal modification and improve the tensile strength and modulus of the polymeric electrospun nanofiber. Ca uploaded through electrospun fibers could provide nucleation sites for the formation of hydroxylapatite (HA) coating. After mineralization, the tensile strength and modulus of HA/PA66/CaCl scaffolds reach up to 41.33 ± 16.17 MPa and 168.59 ± 42.20 MPa, respectively. LIVE/DEAD assay shows that compared with pure PA66 scaffolds, a greater density of viable MC3T3-E1 cells were seen on the HA/PA66/CaCl scaffolds. Cell Counting Kit-8 results indicate that HA/PA66/CaCl scaffolds displays a more favorable ability to promote MC3T3-E1 cell proliferation and growth than that of the other groups with the prolongation of culture time. These results demonstrate that HA/PA66/CaCl scaffolds that structurally and chemically resemble native bone have a good cytocompatibility, and might be a potential candidate for bone tissue engineering. [Figure: see text] Impact statement In this work, the calcium chloride-complexed polyamide66 (PA66/CaCl) hybrid nanofibers were prepared by adding CaCl to the PA66 formic acid solution before electrospinning, and then mineralized by saturated calcium phosphate solution. The findings of this study show that the addition of CaCl could achieve an internal modification, improve the tensile strength and modulus of the polymeric electrospun nanofiber, and provide nucleation sites for biomimetic mineralization. This research provides insight and foundation of application of mineralized PA66/CaCl electrospun scaffolding material that structurally and chemically resembles native extracellular matrix for bone tissue engineering.
本研究旨在设计一种新型的电纺支架材料，其结构和化学性质类似于天然细胞外基质，用于骨组织工程。通过静电纺丝法制备了氯化钙络合聚酰胺 66（PA66/CaCl）和纯 PA66 纳米纤维。扫描电子显微镜、X 射线衍射和傅里叶变换红外（FTIR）光谱用于研究聚合物溶液中存在离子盐对电纺支架的机械性能和其他性能的影响。结果表明，在 PA66 溶液中添加 CaCl 可以实现内部改性，提高聚合物电纺纳米纤维的拉伸强度和模量。通过电纺纤维上传的 Ca 可以为羟基磷灰石（HA）涂层的形成提供成核位点。矿化后，HA/PA66/CaCl 支架的拉伸强度和模量分别达到 41.33±16.17 MPa 和 168.59±42.20 MPa。LIVE/DEAD 检测表明，与纯 PA66 支架相比，HA/PA66/CaCl 支架上观察到更多的活 MC3T3-E1 细胞。细胞计数试剂盒-8 结果表明，随着培养时间的延长，HA/PA66/CaCl 支架比其他组更能促进 MC3T3-E1 细胞的增殖和生长。这些结果表明，在结构和化学性质上类似于天然骨的 HA/PA66/CaCl 支架具有良好的细胞相容性，可能是骨组织工程的潜在候选材料。[图：见正文] 影响陈述 在这项工作中，通过在静电纺丝前将 CaCl 添加到 PA66 甲酸溶液中，制备了氯化钙络合聚酰胺 66（PA66/CaCl）杂化纳米纤维，然后用饱和磷酸钙溶液进行矿化。这项研究的结果表明，添加 CaCl 可以实现内部改性，提高聚合物电纺纳米纤维的拉伸强度和模量，并为仿生矿化提供成核位点。这项研究为结构和化学性质类似于天然细胞外基质的矿化 PA66/CaCl 电纺支架材料在骨组织工程中的应用提供了见解和基础。
