Water Pollution Ecology Laboratory, College of Life Sciences, Wuhan University, Wuhan, 430072, Hubei, China.
Hanjiang River Hydrology and Water Resources Survey Bureau, Hydrology Bureau of the Yangtze River Water Conservancy Committee, Xiang Yang, 441022, Hubei, China.
Ecotoxicol Environ Saf. 2020 Sep 1;200:110747. doi: 10.1016/j.ecoenv.2020.110747. Epub 2020 May 24.
The presence of surfactants may affect the bioavailability of polycyclic aromatic hydrocarbons. A hydroponic experiment was conducted to investigate the response of Hydrocharis dubia (Bl.) Backer to different concentrations of linear alkylbenzene sulfonate (LAS), naphthalene (NAP) and their mixture (0.5, 5, 10, and 20 mg/L) for 14 days and 28 days. The results showed that LAS had a greater toxic effect on H. dubia growth than NAP at treatment concentrations of 0.5-20 mg/L. The combined effect of LAS and NAP was damaging to H. dubia at concentrations of LAS + NAP ≥5 + 5 mg/L. When LAS + NAP ≥10 + 10 mg/L, the underground parts of H. dubia suffered more significant damage than the aboveground parts. Under the treatments with LAS, NAP and their mixture, H. dubia experienced oxidative stress. Soluble proteins and antioxidant enzymes were the main substances protecting H. dubia from LAS stress, and superoxide dismutase (SOD) and peroxidase (POD) were the main protective enzymes. When exposed to NAP, H. dubia growth was stimulated and promoted at the same time. In the short-term treatment (14 d), catalase (CAT) activity was sensitive to NAP stimulation, and soluble proteins and SOD were the main protective substances produced. Soluble sugars, SOD and ascorbate peroxidase (APX) played important protective roles during the longer exposure time (28 d). The physiological response of H. dubia exposed to the combined toxicants was weaker than the response to exposure to individual toxicants. The responses of SOD and CAT activity were positive in the short term (14 d), and these were the main protective enzymes. As the exposure time increased (28 d), the plant antioxidant system responded negatively.
表面活性剂的存在可能会影响多环芳烃的生物利用度。通过水培实验研究了不同浓度的直链烷基苯磺酸钠（LAS）、萘（NAP）及其混合物（0.5、5、10 和 20mg/L）对水葫芦（Hydrocharis dubia (Bl.) Backer）生长的影响，实验时间为 14 天和 28 天。结果表明，在 0.5-20mg/L 的处理浓度下，LAS 对水葫芦的生长毒性大于 NAP。当 LAS+NAP≥5+5mg/L 时，LAS 和 NAP 的混合物对水葫芦的联合效应具有破坏性。当 LAS+NAP≥10+10mg/L 时，水葫芦的地下部分比地上部分受到更严重的损害。在 LAS、NAP 及其混合物的处理下，水葫芦经历了氧化应激。可溶性蛋白质和抗氧化酶是保护水葫芦免受 LAS 胁迫的主要物质，超氧化物歧化酶（SOD）和过氧化物酶（POD）是主要的保护酶。当暴露于 NAP 时，水葫芦的生长同时受到刺激和促进。在短期处理（14d）中，过氧化氢酶（CAT）活性对 NAP 的刺激敏感，可溶性蛋白质和 SOD 是产生的主要保护物质。在较长暴露时间（28d）中，可溶性糖、SOD 和抗坏血酸过氧化物酶（APX）发挥了重要的保护作用。水葫芦暴露于混合毒物时的生理反应比单独暴露于毒物时的反应弱。SOD 和 CAT 活性的短期（14d）响应为阳性，它们是主要的保护酶。随着暴露时间的增加（28d），植物抗氧化系统产生了负响应。
