Water Pollution Ecology Laboratory, College of Life Sciences, Wuhan University, Wuhan, 430072, Hubei, PR China.
Water Pollution Ecology Laboratory, College of Life Sciences, Wuhan University, Wuhan, 430072, Hubei, PR China.
Ecotoxicol Environ Saf. 2020 Oct 1;202:110890. doi: 10.1016/j.ecoenv.2020.110890. Epub 2020 Jun 24.
The presence of tetracycline is ubiquitous and has adverse effects on aquatic systems. A hydroponic experiment was conducted to investigate the ecological sensitivity of Hydrocharis dubia (Bl.) Backer and Trapa bispinosa Roxb. Exposed to different concentrations of tetracycline (0, 0.1, 1, 10, 30 and 50 mg/L) for one day (1D) and 14 days (14D). The results showed that after 1D of tetracycline exposure, the physiological indices of H. dubia had no remarkable change except for proline which was significantly stimulated under 0.1 mg/L tetracycline. For T. bispinosa, guaiacol peroxidase (POD), polyphenol oxidase (PPO) and ascorbate peroxidase (APX) activity and protein and proline content were notably promoted under different concentrations of tetracycline, but PPO activity was significantly decreased in 50 mg/L. After 14D, tetracycline caused no harm to the growth and protein content of H. dubia, but negatively influenced lipid peroxidation product and chlorophyll content in H. dubia under high tetracycline concentrations. Superoxide dismutase (SOD) and POD activity of H. dubia significantly increased at high tetracycline concentrations, while catalase (CAT) and PPO activity significantly decreased. APX activity in H. dubia increased with tetracycline concentrations at low tetracycline concentrations. For T. bispinosa, high concentrations of tetracycline application significantly inhibited its growth and the content of protein and chlorophyll. SOD, POD, CAT, and PPO activity of T. bispinosa were induced under different concentrations of tetracycline and no lipid peroxidation was observed. APX activity in T. bispinosa was significantly inhibited at high tetracycline concentrations. The results suggest that tetracycline can cause oxidative damage in H. dubia but harm the metabolism process of T. bispinosa without inducing oxidative damage. Overall, the sensitivity of T. bispinosa exposed to tetracycline exposure is higher than that of H. dubia.
四环素的存在无处不在，对水生系统有不良影响。本水培实验旨在研究不同浓度四环素（0、0.1、1、10、30 和 50mg/L）暴露一天（1D）和 14 天（14D）对水鳖（Hydrocharis dubia (Bl.) Backer）和菱角（Trapa bispinosa Roxb.）的生态敏感性。结果表明，四环素暴露 1D 后，水鳖的生理指标除在 0.1mg/L 四环素下脯氨酸显著受刺激外，没有明显变化。对于菱角，不同浓度的四环素显著促进了愈创木酚过氧化物酶（POD）、多酚氧化酶（PPO）和抗坏血酸过氧化物酶（APX）活性以及蛋白质和脯氨酸含量，但在 50mg/L 时 PPO 活性显著下降。14D 后，四环素对水鳖的生长和蛋白质含量没有危害，但在高浓度四环素下，会对水鳖的脂质过氧化产物和叶绿素含量产生负面影响。高浓度四环素下水鳖的超氧化物歧化酶（SOD）和 POD 活性显著增加，而过氧化物酶（CAT）和 PPO 活性显著降低。低浓度四环素下水鳖的 APX 活性随四环素浓度的增加而增加。对于菱角，高浓度的四环素应用显著抑制其生长和蛋白质、叶绿素含量。不同浓度的四环素诱导了菱角的 SOD、POD、CAT 和 PPO 活性，未观察到脂质过氧化。高浓度的四环素显著抑制了菱角的 APX 活性。结果表明，四环素会导致水鳖发生氧化损伤，但不会引起菱角的代谢过程受到损害，也不会诱导氧化损伤。总的来说，暴露于四环素的菱角比水鳖更敏感。
