Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
Moscow Institute of Physics and Technology (National Research University), Dolgoprudny, 141700, Russia.
Mech Ageing Dev. 2020 Jul;189:111260. doi: 10.1016/j.mad.2020.111260. Epub 2020 May 24.
Fucoxanthin, as a main marine carotenoid, exhibit a wide variety of bioactivities, including antioxidant activity. Previously, we have shown the geroprotective activity of fucoxanthin on Drosophila and C. elegans. Our new study aimed to compare the antioxidant activity of fucoxanthin in early and late passage normal human cells LECh4(81) in physiological conditions and under oxidative stress. In addition, using the RNA-seq we have analyzed the transcriptomic changes during the replicative senescence of fibroblasts treated with fucoxanthin. Results showed that fucoxanthin at a concentration of 5 μM caused the most pronounced antioxidant effect in the late passage cells. Moreover, transcriptomic data showed the increased expression levels of genes related to the Nrf2/ARE pathway. According to the analysis of enriched KEGG pathways, fucoxanthin altered cellular processes like ribosome biogenesis, lipid metabolism, and cell cycle regulation including some age-related pathways such as Wnt, JAK-STAT, and FoxO signaling pathways. We suggest that fucoxanthin may have therapeutic potential for treating age-related diseases.
岩藻黄质是一种主要的海洋类胡萝卜素，具有广泛的生物活性，包括抗氧化活性。此前，我们已经证明了岩藻黄质对果蝇和秀丽隐杆线虫的抗衰老活性。我们的新研究旨在比较岩藻黄质在生理条件下和氧化应激下对早期和晚期 passage 正常人类细胞 LECh4(81)的抗氧化活性。此外，我们还使用 RNA-seq 分析了用岩藻黄质处理的成纤维细胞复制性衰老过程中的转录组变化。结果表明，在晚期 passage 细胞中，浓度为 5μM 的岩藻黄质引起了最显著的抗氧化作用。此外，转录组数据显示，与 Nrf2/ARE 途径相关的基因表达水平增加。根据富集的 KEGG 途径分析，岩藻黄质改变了细胞过程，如核糖体生物发生、脂质代谢和细胞周期调节，包括一些与年龄相关的途径，如 Wnt、JAK-STAT 和 FoxO 信号通路。我们认为岩藻黄质可能具有治疗与年龄相关疾病的潜力。
