NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE).
J Gen Appl Microbiol. 2020 Sep 30;66(4):215-219. doi: 10.2323/jgam.2019.10.001. Epub 2020 May 26.
An aerobic bacterium, designated strain 5N-3 (NBRC 113055), that degrades cis-dichloroethene (cDCE) was isolated from a sea sediment in Japan. Strain 5N-3 was able to degrade a certain amount of cDCE in the presence of pyruvate without the action of inducers. In the presence of inducers, such as phenol and benzene, the strain completely removed cDCE. By the application of 16S ribosomal RNA (16S rRNA) gene sequencing and average nucleotide identity analyses, the strain 5N-3 was identified as Marinobacter salsuginis. On the other hand, identified species of Marinobacter are not known to degrade cDCE at all. A draft genome sequence analysis of the strain 5N-3 suggested that the dmp-homologous operon (operon for phenol degradation) may be contributing to the aerobic degradation of cDCE. This is the first report on an aerobic marine bacterium that has been found to degrade cDCE.
从日本的海泥中分离到一株能够降解顺式-二氯乙烯（cDCE）的需氧细菌，命名为 5N-3 株（NBRC 113055）。在没有诱导物存在的情况下，5N-3 株能够在丙酮酸的存在下降解一定量的 cDCE。在有诱导物（如苯酚和苯）存在的情况下，该菌株能完全去除 cDCE。通过 16S 核糖体 RNA（16S rRNA）基因测序和平均核苷酸同一性分析，将菌株 5N-3 鉴定为 Marinobacter salsuginis。另一方面，已知的 Marinobacter 种根本不能降解 cDCE。对 5N-3 株的基因组草图序列分析表明，dmp 同源操纵子（苯酚降解操纵子）可能有助于 cDCE 的好氧降解。这是第一份关于发现能降解 cDCE 的好氧海洋细菌的报告。
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