Early A E, Williams J G
Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, UK.
Development. 1988 Jul;103(3):519-24. doi: 10.1242/dev.103.3.519.
One important role of DIF, the stalk cell-specific inducer of Dictyostelium, may be to divert cells from the spore cell pathway of differentiation. The D19 gene encodes an mRNA which is highly enriched in prespore over prestalk cells in the migratory slug. We show, using a mutant defective in DIF accumulation, that the concentration of D19, and several other prespore mRNA sequences, decreases in the presence of exogenous DIF. There is evidence that both transcriptional and post-transcriptional controls operate to regulate expression of these genes. We have performed in vitro nuclear transcription and mRNA half-life analyses, and find that DIF acts at the transcriptional level to repress the accumulation of the D19 mRNA.
盘基网柄菌柄细胞特异性诱导因子DIF的一个重要作用可能是使细胞从孢子细胞分化途径转向。D19基因编码一种mRNA，在迁移期蛞蝓体中，前孢子细胞中的这种mRNA比前柄细胞中高度富集。我们利用一个DIF积累存在缺陷的突变体表明，在外源DIF存在的情况下，D19以及其他几种前孢子mRNA序列的浓度会降低。有证据表明，转录和转录后控制都参与调控这些基因的表达。我们进行了体外细胞核转录和mRNA半衰期分析，发现DIF在转录水平上起作用，抑制D19 mRNA的积累。
