Department of Chemistry and Chemical Processes, Tunceli Vocation School, Munzur University, 62000, Tunceli, Turkey.
Department of Veterinary Medicine, Laboratorian and Veterinarian Health Programme, Pertek Sakine Genc Vocation School, Munzur University, 62000, Tunceli, Turkey.
Curr Microbiol. 2020 Jul;77(7):1301-1307. doi: 10.1007/s00284-020-02042-y.
The research study was about revealing the biochemical response of Gammarus pulex related to insecticide methomyl before and after bioremediation by two soil bacteria species, Ochrobactrum thiophenivorans and Sphingomonas melonis. Catalase (CAT), glutathione S-transferase.(GST), cytochrome. P4501A1 (CYP1A1) activities in G. Pulex related to methomyl solution were investigated in 24 h and 96 h. ELISA method was used for test studies. CAT enzyme was decreased in Gammarus pulex that was exposed to methomyl after all exposure period (P < 0.05). CAT activities were returned to control results after bioremediation assays. GST enzyme activity was decreased depending on methomyl exposure during 24 h but increased during 4 days (P < 0.05). After 8 days of bioremediation period, GST activity increased again during 24 h while decreased during 4 days (P < 0.05). CYP1A1 activity increased in Gammarus pulex that was exposed to methomyl after all exposure period (P > 0.05). After bioremediation, statistically significant changes were not revealed in CYP1A1 activities (P > 0.05). According to the results of our study, CYP1A1, CAT, and GST activities in G. pulex sanctioned the capability of Ochrobactrum thiophenivorans and Sphingomonas melonis in methomyl bioremediation. Isolated and enriched Ochrobactrum thiophenivorans and Sphingomonas melonis that were added to 2.5 ppb concentrations of methomyl for 8 days. Each day, chemical oxygen demand (COD) and biochemical oxygen demand (BOD), pH and dissolved oxygen parameters were monitored. At the final phase of the bioremediation step, it was determined that these bacteria have efficient methomyl bioremediation properties in a mixed corsortia at a rate of 86%. These results show that these bacteria can be used for bioremediate the receiving environments that are polluted by these kinds of insecticides.
这项研究旨在揭示食蚊鱼在两种土壤细菌（Ochrobactrum thiophenivorans 和 Sphingomonas melonis）生物修复前后与杀虫剂灭多威相关的生化反应。在 24 小时和 96 小时内，研究了与灭多威溶液相关的食蚊鱼中的过氧化氢酶 (CAT)、谷胱甘肽 S-转移酶 (GST) 和细胞色素 P4501A1 (CYP1A1) 活性。采用 ELISA 法进行测试研究。暴露于灭多威后的所有暴露期，食蚊鱼中的 CAT 酶均下降（P<0.05）。生物修复试验后，CAT 活性恢复至对照结果。暴露于灭多威 24 小时内，GST 酶活性下降，但在 4 天内增加（P<0.05）。在生物修复 8 天后，GST 活性在 24 小时内再次增加，而在 4 天内下降（P<0.05）。暴露于灭多威后，食蚊鱼中的 CYP1A1 活性增加，所有暴露期均如此（P>0.05）。生物修复后，CYP1A1 活性未发生统计学显著变化（P>0.05）。根据我们的研究结果，食蚊鱼中的 CYP1A1、CAT 和 GST 活性证明了 Ochrobactrum thiophenivorans 和 Sphingomonas melonis 对灭多威的生物修复能力。分离并富集了添加到 2.5ppb 灭多威浓度中 8 天的 Ochrobactrum thiophenivorans 和 Sphingomonas melonis。每天监测化学需氧量 (COD) 和生化需氧量 (BOD)、pH 值和溶解氧参数。在生物修复阶段的最后阶段，确定这些细菌在混合菌群中以 86%的速率对灭多威具有有效的生物修复特性。这些结果表明，这些细菌可用于生物修复受此类杀虫剂污染的受纳环境。
