Fekete D M, Brockes J P
MRC Cell Biophysics Unit, London, UK.
Development. 1988 Jul;103(3):567-73. doi: 10.1242/dev.103.3.567.
Adult urodele amphibians can regenerate their limbs after amputation by a process that requires the presence of axons at the amputation plane. Paradoxically, if the limb develops in the near absence of nerves (the 'aneurogenic' limb) it can subsequently regenerate in a nerve-independent fashion. The growth zone (blastema) of regenerating limbs normally contains progenitor cells whose division is nerve-dependent. A monoclonal antibody that marks these nerve-dependent cells in the normal blastema does not stain the mesenchymal cells of developing limb buds and only stains the amputated limb bud when axons have reached the plane of amputation. This report shows that the blastemal cells of the regenerating aneurogenic limb also fail to react with the antibody in situ. These data suggest that the blastemal cells arising during normal regeneration have been altered by the nerve. This regulation may occur either at the time of amputation (when the antigen is expressed) or during development (when the limb is first innervated).
成年有尾两栖动物在截肢后能够通过一个需要在截肢平面存在轴突的过程来再生其肢体。矛盾的是，如果肢体在几乎没有神经的情况下发育（“无神经支配”肢体），它随后能够以不依赖神经的方式再生。再生肢体的生长区（芽基）通常含有祖细胞，其分裂依赖于神经。一种在正常芽基中标记这些神经依赖细胞的单克隆抗体不会对发育中的肢芽间充质细胞染色，并且只有当轴突到达截肢平面时才会对截肢后的肢芽染色。本报告表明，再生的无神经支配肢体的芽基细胞在原位也不能与该抗体发生反应。这些数据表明，正常再生过程中产生的芽基细胞已被神经改变。这种调节可能发生在截肢时（当抗原表达时）或发育过程中（当肢体首次接受神经支配时）。
