Dyck Papa K Van, Hockaden Natasha, Nelson Emma C, Koch Alyssa R, Hester Kamil L, Pillai Neil, Coffing Gabrielle C, Burns Alan R, Lafontant Pascal J
Department of Biology, DePauw University, Greencastle, IN 46135, USA.
College of Optometry, University of Houston, Houston, TX 77204, USA.
J Cardiovasc Dev Dis. 2020 Oct 3;7(4):41. doi: 10.3390/jcdd7040041.
In the last two decades, the zebrafish has emerged as an important model species for heart regeneration studies. Various approaches to model loss of cardiac myocytes and myocardial infarction in the zebrafish have been devised, and have included resection, genetic ablation, and cryoinjury. However, to date, the response of the zebrafish ventricle to cautery injury has not been reported. Here, we describe a simple and reproducible method using cautery injury via a modified nichrome inoculating needle as a probe to model myocardial infarction in the zebrafish ventricle. Using light and electron microscopy, we show that cardiac cautery injury is attended by significant inflammatory cell infiltration, accumulation of collagen in the injured area, and the reconstitution of the ventricular myocardium. Additionally, we document the ablation of cardiac nerve fibers, and report that the re-innervation of the injured zebrafish ventricle is protracted, compared to other repair processes that accompany the regeneration of the cauterized ventricle. Taken together, our study demonstrates that cautery injury is a simple and effective means for generating necrotic tissue and eliciting a remodeling and regenerative response in the zebrafish heart. This approach may serve as an important tool in the methods toolbox for regeneration studies in the zebrafish.
在过去二十年中，斑马鱼已成为心脏再生研究的重要模式生物。人们设计了多种在斑马鱼中模拟心肌细胞损失和心肌梗死的方法，包括切除、基因消融和冷冻损伤。然而，迄今为止，尚未有关于斑马鱼心室对烧灼损伤反应的报道。在此，我们描述一种简单且可重复的方法，即使用经改良的镍铬合金接种针作为探针进行烧灼损伤，以模拟斑马鱼心室的心肌梗死。通过光学和电子显微镜观察，我们发现心脏烧灼损伤伴随着大量炎性细胞浸润、损伤区域胶原积累以及心室心肌的重构。此外，我们记录了心脏神经纤维的消融，并报告与烧灼心室再生伴随的其他修复过程相比，损伤的斑马鱼心室重新神经支配的过程较为漫长。综上所述，我们的研究表明，烧灼损伤是在斑马鱼心脏中产生坏死组织并引发重塑和再生反应的一种简单有效的方法。这种方法可能成为斑马鱼再生研究方法工具箱中的重要工具。
