Department of Plant Pathology and Microbiology, Iowa State University, Ames, IA, USA.
Methods Mol Biol. 2020;2139:147-156. doi: 10.1007/978-1-0716-0528-8_11.
Proteins produce or regulate nearly every component of cells. Thus, the ability to quantitatively determine the protein abundance and posttranslational modification (PTM) state is a critical aspect toward our understanding of biological processes. In this chapter, we describe methods to globally quantify protein abundance and phosphorylation state using isobaric labeling with tandem mass tags followed by phosphopeptide enrichment.
蛋白质产生或调节细胞的几乎每一个组成部分。因此，定量测定蛋白质丰度和翻译后修饰（PTM）状态的能力是我们理解生物过程的一个关键方面。在本章中，我们描述了使用串联质量标签进行等重标记，然后进行磷酸肽富集，来全局定量测定蛋白质丰度和磷酸化状态的方法。
