Department of Chemistry, Indian Institution of Technology Roorkee, Roorkee, 247667, Uttarakhand, India.
Department of Biotechnology, Indian Institution of Technology Roorkee, Roorkee, 247667, Uttarakhand, India.
Chemistry. 2020 Nov 26;26(66):15150-15158. doi: 10.1002/chem.202002048. Epub 2020 Oct 6.
Gold supra-pyramid structures were obtained by the addition of acidic solution of cucurbit[8]uril (CB[8]) to an aqueous solution of citrate stabilized gold nanoparticles (AuNP). The reaction resulted in the precipitation of supra-pyramid from the solution after just 1 min of shaking. Microscopic images confirmed formation of the supra-pyramid. The stepwise structural transformation towards the supra-pyramid was examined with variable concentrations of CB[8] to AuNP solution. Anionic counter parts of these acids (Br , NO , SO and Cl ) controlled the size of the synthesized supra-pyramids. These supra-pyramid hosts showed uptake of three anticancer drugs: oral drugs etoposide, prednisolone and intravenous drug doxorubicin. Releases of drugs from these hosts were emulated at acidic stomach pH, basic small intestinal pH and in the presence of human serum albumin (HSA). The specific release of doxorubicin was confirmed at small intestinal pH 7.4. Poor release of drugs in presence of CB[8] specific guest 1-adamantanamine confirmed the role of the supra-pyramid as the exclusive host. The release of doxorubicin from the supra-pyramid at pH 7.4 was confirmed by fluorescence microscopic imaging with prostate cancer DU-145 cell line.
金超金字塔结构是通过将酸性的葫芦脲[8]（CB[8]）溶液添加到柠檬酸稳定的金纳米粒子（AuNP）的水溶液中得到的。反应仅在摇晃 1 分钟后，超金字塔就从溶液中沉淀出来。显微镜图像证实了超金字塔的形成。通过改变 CB[8]与 AuNP 溶液的浓度，研究了超金字塔的逐步结构转变。这些酸的阴离子对应物（Br 、NO 、SO 和 Cl ）控制了合成的超金字塔的大小。这些超金字塔主体显示出对三种抗癌药物的摄取：口服药物依托泊苷、泼尼松龙和静脉注射药物阿霉素。在酸性胃 pH 值、碱性小肠 pH 值和人血清白蛋白（HSA）存在下模拟了这些主体中药物的释放。在小肠 pH 值 7.4 下证实了阿霉素的特异性释放。在 CB[8]特异性客体 1-金刚烷胺存在下药物释放不佳，证实了超金字塔作为唯一主体的作用。荧光显微镜成像证实了阿霉素在 pH 值 7.4 时从超金字塔中的释放，使用前列腺癌 DU-145 细胞系。
