Barooah Nilotpal, Kunwar Amit, Khurana Raman, Bhasikuttan Achikanath C, Mohanty Jyotirmayee
Radiation & Photochemistry Division, Bhabha Atomic Research Centre, Mumbai, 400 085, India.
Homi Bhabha National Institute, Training School Complex, Anushaktinagar, Mumbai, 400 094, India.
Chem Asian J. 2017 Jan 3;12(1):122-129. doi: 10.1002/asia.201601411. Epub 2016 Dec 9.
We report the construction of a non-toxic nanoassembly of bovine serum albumin (BSA) protein and the cucurbit[7]uril macrocycle as well as its stimuli-responsive breakage with adamantylamine or pH, which restores the protein structure and recognition properties. The assembly showed efficient loading and controlled release of a standard drug, doxorubicin (DOX), and the same was validated in live cells. The cell viability studies documented that the DOX-loaded assembly mask the cytotoxicity of DOX and the toxicity can be revived at the target on demand, triggering its therapeutic activation. This is found to be more effective in the cancer cells. In addition, such host-assisted protein assemblies are also highly promising for stabilizing/protecting the native protein structure, a viable approach to prevent/inhibit protein misfolding and aggregation.
我们报道了牛血清白蛋白（BSA）蛋白与葫芦[7]脲大环构建的无毒纳米组装体，以及其与金刚烷胺或pH的刺激响应性断裂，这可恢复蛋白质结构和识别特性。该组装体显示出对标准药物阿霉素（DOX）的高效负载和控释，并在活细胞中得到了验证。细胞活力研究表明，负载DOX的组装体掩盖了DOX的细胞毒性，并且毒性可在靶点按需恢复，触发其治疗激活。这在癌细胞中被发现更有效。此外，这种宿主辅助的蛋白质组装体在稳定/保护天然蛋白质结构方面也非常有前景，是预防/抑制蛋白质错误折叠和聚集的可行方法。
