Arturo-Terranova Daniela, Giraldo-Ocampo Sebastián, Castillo Andrés
Departamento de Biología, Facultad de Ciencias Naturales y Exactas, Universidad del Valle, Cali, Colombia; Posgrado en Ciencias Biomédicas, Escuela de Ciencias Básicas, Facultad de Salud, Universidad del Valle, Cali, Colombia.
Posgrado en Ciencias Biomédicas, Escuela de Ciencias Básicas, Facultad de Salud, Universidad del Valle, Cali, Colombia.
Biomedica. 2020 May 1;40(Supl. 1):76-88. doi: 10.7705/biomedica.4917.
Introduction: The Epstein-Barr virus (EBV) is an ubiquitous and oncogenic virus associated with the development of diseases such as infectious mononucleosis, Burkitt’s lymphoma, nasopharyngeal carcinoma, and other neoplasms. Currently, two types are recognized: EBV-1 and EBV-2, which have genetic differences with their EBNA nuclear antigens. Likewise, due to the high degree of heterogeneity and variability found in the LMP1 protein of the virus, variants associated with pathogenesis or specific geographic regions have been described. Objective: To identify and characterize molecularly EBV variants detected in the oral cavity of 84 adolescents in Cali, Colombia. Materials and methods: Conventional PCR amplification, purification, and sequencing of the gen EBNA3C were carried out to typify the virus and the C-ter domain of the LMP1 protein to identify variants. We also conducted a phylogenetic and nucleotide variant analysis of the obtained sequences versus pathogenic or geographic variants reported in GenBank-NCBI. Results: The predominant viral subtype was EBV-1 (79%); 72.6% was grouped with the pathogenic variant Raji, derived from B lymphocytes of a patient with Burkitt›s lymphoma, 13.7% was related to a variant of Mediterranean origin, and 13.7% was not grouped with any of the reference variants. Conclusions: This is the first time that variants of LMP1-EBV have been identified in Cali, Colombia. Additional studies are necessary to characterize the unidentified variant and to determine if it is pathogenic or if it is just an isolate present in the city of Cali.
爱泼斯坦-巴尔病毒（EBV）是一种普遍存在的致癌病毒，与传染性单核细胞增多症、伯基特淋巴瘤、鼻咽癌及其他肿瘤等疾病的发生有关。目前，已识别出两种类型：EBV-1和EBV-2，它们在EBNA核抗原方面存在基因差异。同样，由于在该病毒的LMP1蛋白中发现高度的异质性和变异性，已描述了与发病机制或特定地理区域相关的变体。目的：对在哥伦比亚卡利市84名青少年口腔中检测到的EBV变体进行分子鉴定和特征分析。材料和方法：对EBNA3C基因进行常规PCR扩增、纯化及测序以对病毒进行分型，并对LMP1蛋白的C端结构域进行测序以识别变体。我们还对获得的序列与GenBank-NCBI中报道的致病或地理变体进行了系统发育和核苷酸变体分析。结果：主要的病毒亚型为EBV-1（79%）；72.6%与源自伯基特淋巴瘤患者B淋巴细胞的致病变体Raji归为一组，13.7%与地中海起源的变体相关，13.7%未与任何参考变体归为一组。结论：这是首次在哥伦比亚卡利市识别出LMP1-EBV变体。需要进行更多研究以表征未识别的变体，并确定其是否致病或只是卡利市存在的一种分离株。
