Cancer Biology Program, Fox Chase Cancer Center, Philadelphia, PA 19111.
Department of Biochemistry, University of Washington, Seattle, Washington 98195.
Mol Biol Cell. 2020 Jun 1;31(12):1201-1205. doi: 10.1091/mbc.E18-10-0675.
Many different enzymes in intermediate metabolism dynamically assemble filamentous polymers in cells, often in response to changes in physiological conditions. Most of the enzyme filaments known to date have only been observed in cells, but in a handful of cases structural and biochemical studies have revealed the mechanisms and consequences of assembly. In general, enzyme polymerization functions as a mechanism to allosterically tune enzyme kinetics, and it may play a physiological role in integrating metabolic signaling. Here, we highlight some principles of metabolic filaments by focusing on two well-studied examples in nucleotide biosynthesis pathways-inosine-5'-monophosphate (IMP) dehydrogenase and cytosine triphosphate (CTP) synthase.
细胞内许多不同的中间代谢酶在生理条件变化时会动态组装成丝状聚合物。到目前为止，人们所知道的大多数酶丝仅在细胞中观察到，但在少数情况下，结构和生化研究揭示了组装的机制和后果。一般来说，酶聚合作为一种变构调节酶动力学的机制，它可能在整合代谢信号中发挥生理作用。在这里，我们通过关注核苷酸生物合成途径中的两个研究较好的例子——肌苷-5'-单磷酸（IMP）脱氢酶和胞嘧啶三磷酸（CTP）合酶，强调代谢丝的一些原理。
