Center for Immunization and Infection Research in Cancer (CIIRC), H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, 33612, USA.
H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, 33612, USA; Statistical Center for HIV/AIDS Research and Prevention (SCHARP), Fred Hutchinson Cancer Research Center, Seattle, WA, 98109, USA.
Papillomavirus Res. 2020 Jun;9:100199. doi: 10.1016/j.pvr.2020.100199. Epub 2020 May 25.
Oral human papillomavirus (HPV) attributable oropharyngeal cancers are on the rise in many countries. Oral HPV infections among healthy individuals are commonly detected using oral gargle samples. However, the optimal method for HPV genotyping oral gargle specimens in research studies has not been previously evaluated.
Oral gargle samples from 1455 HPV Infection in Men (HIM) study participants were HPV genotyped using two different methods: Linear Array and the SPF PCR-DEIA-LiPA. The sensitivity of the two tests for detecting individual HPV types and grouped HPV types, high-risk HPV, low-risk HPV, grouped 4-HPV-vaccine types, and grouped 9-HPV-vaccine-types, and the degree of concordance between the two tests was assessed. We also examined whether socio-demographic-behavioral factors were associated with concordance between the two assays.
The sensitivity of SPF PCR-DEIA-LiPA was higher than Linear Array, with the exception of HPV 70, for the detection of oral HPV. The prevalence ratio of SPF PCR-DEIA-LiPA to Linear Array varied between 1.0 and 9.0 for individual HPV genotypes, excluding HPV 70, and between 3.8 and 4.4 for grouped 4-valent and 9-valent HPV vaccine types, respectively. There was no association between socio-demographic-behavioral factors and discordance in results between the two tests for oral HPV 16 detection.
SPF PCR-DEIA-LiPA was more sensitive than Linear Array for detecting HPV in oral gargle samples. Given the growing importance of detecting oral HPV infection for research studies of oral HPV natural history and vaccine effectiveness evaluation, we recommend using methods with higher sensitivity such as SPF PCR-DEIA-LiPA for detecting HPV in oral gargle samples.
在许多国家，口腔人乳头瘤病毒（HPV）相关的口咽癌发病率正在上升。使用口腔漱口样本通常可以检测到健康个体中的口腔 HPV 感染。然而，之前尚未评估研究中用于 HPV 基因分型口腔漱口标本的最佳方法。
对 1455 名男性 HPV 感染研究（HIM）参与者的口腔漱口样本使用两种不同的方法进行 HPV 基因分型：线性阵列和 SPF PCR-DEIA-LiPA。评估了两种检测方法对检测个别 HPV 类型和分组 HPV 类型、高危 HPV、低危 HPV、分组 4 价 HPV 疫苗类型和分组 9 价 HPV 疫苗类型的灵敏度，以及两种检测方法之间的一致性程度。我们还检查了社会人口统计学行为因素是否与两种检测方法之间的一致性相关。
SPF PCR-DEIA-LiPA 的灵敏度高于线性阵列，除 HPV 70 外，用于检测口腔 HPV。SPF PCR-DEIA-LiPA 与线性阵列的比值在 1.0 到 9.0 之间，个体 HPV 基因型除外 HPV 70，分组 4 价和 9 价 HPV 疫苗类型分别为 3.8 到 4.4。社会人口统计学行为因素与两种检测方法在口腔 HPV 16 检测结果中的不一致性之间没有关联。
SPF PCR-DEIA-LiPA 检测口腔漱口样本中的 HPV 比线性阵列更敏感。鉴于检测口腔 HPV 感染对于口腔 HPV 自然史和疫苗有效性评估的研究越来越重要，我们建议使用灵敏度更高的方法，如 SPF PCR-DEIA-LiPA，用于检测口腔漱口样本中的 HPV。
