Grain and Oil Food Processing Key Laboratory of Jilin Province, Jilin Business and Technology College, Changchun 130507, China.
College of Animal Sciences/ College of Life Science, Yangtze University, Jingzhou 434023, China.
Food Chem. 2020 Oct 15;327:127058. doi: 10.1016/j.foodchem.2020.127058. Epub 2020 May 18.
A "turn-on" fluorescence sensor was developed for total detection of fumonisin B, B and B (FB, FB and FB) in maize samples. Rhodamine B isothiocyanate (RBITC) and gold nanoparticles (AuNPs) were employed as the energy donor-acceptor pairs. The intensity of restored fluorescence was inversely proportional to the concentration of the FBs. The limit of detection (LOD) of the sensor for FB was 23.80 pg/mL. The coefficient of variation (CV) was both less than 7% for intra- and inter-assay. The linear range was from 51.39 to 2125.92 pg/mL. The average recoveries of FBs from maize samples were ranged from 88.7% to 107.2%. The correlation coefficient of the results between the developed sensor and LC-MS/MS was 0.9970. To fulfill the procedure of the assay, only 46 min was needed. These results suggest that the fluorescence "turn-on" sensor has great potential applications in the analysis of FBs in maize samples.
一种“开启”型荧光传感器被开发出来，用于总检测玉米样品中的伏马菌素 B、B 和 B（FB、FB 和 FB）。罗丹明 B 异硫氰酸酯（RBITC）和金纳米粒子（AuNPs）被用作能量供体-受体对。恢复荧光的强度与 FB 的浓度成反比。该传感器对 FB 的检测限（LOD）为 23.80 pg/mL。内和间分析的变异系数（CV）均小于 7%。线性范围为 51.39 至 2125.92 pg/mL。从玉米样品中 FB 的平均回收率范围为 88.7%至 107.2%。开发的传感器与 LC-MS/MS 之间结果的相关系数为 0.9970。完成测定程序仅需 46 分钟。这些结果表明，荧光“开启”传感器在玉米样品中 FB 的分析中具有很大的应用潜力。
