Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand.
Analytical Chemistry and Environment Research Unit, Division of Chemistry, Department of Science, Faculty of Science and Technology, Prince of Songkla University, Pattani, 94000, Thailand.
Mikrochim Acta. 2019 Aug 28;186(9):655. doi: 10.1007/s00604-019-3778-x.
A colorimetric method was developed for the determination of the mold toxin fumonisin B1 (FB1). It is based on the aggregation of cysteamine-capped gold nanoparticles (Cys-AuNPs). The assay involves alkaline hydrolysis of FB1 to obtain hydrolyzed fumonisin B1 (HFB1). The latter induces the aggregation of Cys-AuNPs which results in a color change from wine-red to blue-gray, best at a pH value of 9.0. A plot of absorbance ratio at 645/520 nm versus FB1 concentration is linear in the 2-8 μg kg FB1 concentration range, and the detection limit is 0.90 μg kg. Inter-day and intra-day precisions are <6.2%, and recoveries from spiked samples ranged from 93 to 99%. The assay was successfully applied to the determination of FB1 in corn samples. It has a high selectivity over other competitive mycotoxins including aflatoxin, zearalenone, citrinin and patulin. The method is more selective than the detection of FB1 directly which may lead to false-positive errors. Graphical abstract Schematic representation of colorimetric assay of fumonisin B1 (FB1). FB1 was alkali-hydrolyzed and its product (hydrolyzed fumonisin B1) induces cysteamine-capped gold nanoparticles (Cys-AuNPs) via hydrogen bondings. The aggregation of Cys-AuNPs causes changes in color from wine-red to blue-gray.
建立了一种比色法测定真菌毒素伏马菌素 B1（FB1）的方法。该方法基于半胱氨酸修饰的金纳米粒子（Cys-AuNPs）的聚集。该测定法涉及 FB1 的碱性水解以获得水解伏马菌素 B1（HFB1）。后者诱导 Cys-AuNPs 的聚集，导致颜色从酒红色变为蓝灰色，最佳 pH 值为 9.0。在 2-8μgkg FB1 浓度范围内，吸光度比值（645/520nm）与 FB1 浓度的关系呈线性，检测限为 0.90μgkg。日内和日间精密度均<6.2%，加标样品的回收率在 93%至 99%之间。该测定法成功应用于玉米样品中 FB1 的测定。与其他竞争真菌毒素（包括黄曲霉毒素、玉米赤霉烯酮、桔青霉素和展青霉素）相比，该方法具有更高的选择性。与直接检测 FB1 相比，该方法的选择性更高，这可能导致假阳性错误。
