Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Solnavägen, SE-171 77 Stockholm, Sweden.
S3i, LLC, Reisterstown, MD, USA.
Ann Work Expo Health. 2020 Oct 8;64(8):852-865. doi: 10.1093/annweh/wxaa053.
Detecting infectious aerosols is central for gauging and countering airborne threats. In this regard, the Coriolis® µ cyclonic air sampler is a practical, commercial collector that can be used with various analysis methods to monitor pathogens in air. However, information on how to operate this unit under optimal sampling and biosafety conditions is limited. We investigated Coriolis performance in aerosol dispersal experiments with polystyrene microspheres and Bacillus globigii spores. We report inconsistent sample recovery from the collector cone due to loss of material when sampling continuously for more than 30 min. Introducing a new collector cone every 10 min improved this shortcoming. Moreover, we found that several surfaces on the device become contaminated during sampling. Adapting a high efficiency particulate air-filter system to the Coriolis prevented contamination without altering collection efficiency or tactical deployment. A Coriolis modified with these operative and technical improvements was used to collect aerosols carrying microspheres released inside a Biosafety Level-3 laboratory during simulations of microbiological spills and aerosol dispersals. In summary, we provide operative and technical solutions to the Coriolis that optimize microbiological air sampling and improve biosafety.
检测传染性气溶胶对于评估和应对空气传播威胁至关重要。在这方面，Coriolis®µ气旋式空气采样器是一种实用的商用收集器，可与各种分析方法一起用于监测空气中的病原体。然而，关于如何在最佳采样和生物安全条件下操作该设备的信息有限。我们用聚苯乙烯微球和枯草芽孢杆菌孢子进行了气溶胶扩散实验，研究了 Coriolis 的性能。我们报告称，由于连续采样超过 30 分钟时材料损失，从收集器锥体中回收的样本不一致。每 10 分钟更换一个新的收集器锥体可以改善这一缺点。此外，我们发现设备上的几个表面在采样过程中会被污染。在 Coriolis 上采用高效空气颗粒过滤器系统可以防止污染，而不会改变收集效率或战术部署。经过改进的 Coriolis 可用于在模拟微生物溢出和气溶胶扩散期间在生物安全三级实验室内部释放微球的气溶胶进行采样。总之，我们为 Coriolis 提供了操作和技术解决方案，以优化微生物空气采样并提高生物安全性。
