Institute of Environmental Health, College of Public Health, National Taiwan University, Taipei, Taiwan, Republic of China.
Center for Research on Environmental and Occupational Health, National Taiwan University, Taipei, Taiwan, Republic of China.
Indoor Air. 2019 May;29(3):380-389. doi: 10.1111/ina.12535. Epub 2019 Jan 21.
This study assessed the collection efficiency (CE) of two popularly used sampling devices (BioSampler and Coriolis sampler) for fungal aerosols. Phosphate-buffered saline (PBS) supplemented with or without surfactant (Tween-20, Tween-80, or Triton X-100) and antifoam agent was prepared and used as collection liquids. The agar impactor (BioStage) was simultaneously operated with liquid-based samplers to collect fungi from seven sites located at a university building, public library, and animal farming. Fungal concentrations determined by liquid samplers were divided by those by BioStage, and the ratio values represented CE. Results indicate that the CE of BioSampler was superior to that of Coriolis (P = 0.0001) and the PBS containing surfactant collected fungi better than that without surfactant (P < 0.0001), whereas antifoam agent showed no influence (P = 0.8). Moreover, fungal concentrations determined by BioSampler with surfactant-added PBS were statistically indifferent from those by BioStage (P > 0.05) with a Spearman correlation coefficient of 0.81-0.83 (P < 0.01). In addition to sampler and collection liquid, sampling location was also identified as a significant CE factor (P = 0.006), implying potential influences by fungal genera in the studied fields. Overall, BioSampler with surfactant-supplemented PBS (eg, Triton X-100) is recommended considering the great CE and compatibility with a variety of analytical assays.
本研究评估了两种常用采样器（BioSampler 和 Coriolis 采样器）对真菌气溶胶的收集效率（CE）。制备了含或不含表面活性剂（吐温 20、吐温 80 或 Triton X-100）和消泡剂的磷酸盐缓冲盐水（PBS）作为收集液。同时用琼脂撞击器（BioStage）与液体采样器一起从位于大学建筑、公共图书馆和动物养殖场的七个地点收集真菌。通过液体采样器确定的真菌浓度除以 BioStage 的浓度，比值代表 CE 值。结果表明，BioSampler 的 CE 值优于 Coriolis（P = 0.0001），且含有表面活性剂的 PBS 比不含表面活性剂的 PBS 收集的真菌更好（P < 0.0001），而消泡剂则没有影响（P = 0.8）。此外，添加表面活性剂的 PBS 的 BioSampler 确定的真菌浓度与 BioStage 之间无统计学差异（P > 0.05），Spearman 相关系数为 0.81-0.83（P < 0.01）。除了采样器和收集液外，采样地点也被确定为一个重要的 CE 因素（P = 0.006），这表明研究领域中的真菌属可能会产生潜在影响。总体而言，考虑到高 CE 值和与多种分析测定法的兼容性，建议使用添加了表面活性剂的 PBS 的 BioSampler（例如 Triton X-100）。
