Han Shi Huan, Lee JiUn, Lee Kyung Mee, Jin Yuan Zhe, Yun Hui-Suk, Kim GeunHyung, Lee Jae Hyup
Department of Orthopedic Surgery, College of Medicine, Seoul National University, Seoul, 03080, South Korea; Department of Orthopedic Surgery, YanBian University Hospital, 133000, Yanji, Jilin Province, China.
Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon, South Korea.
J Mech Behav Biomed Mater. 2020 Aug;108:103782. doi: 10.1016/j.jmbbm.2020.103782. Epub 2020 Apr 16.
In this paper, we mainly to evaluate the newly formed bone using the Calcium deficient hydroxyapatite (CDHA)/collagen-based bio-ceramic scaffold as Bone Morphogenetic Protein-2 (BMP-2) carrier in rat calvarial critical-sized bone defect. In the real-time PCR analysis, the CDHA/collagen scaffold loaded rhBMP-2 group showed significantly enhanced results of bone-related gene expression (p < 0.05). In the in vivo study, the micro-CT showed that the main bone formation parameters of percent bone volume and trabecular number of the two experiment groups (CDHA/Collagen (CDHA) group, BV/TV: 14.21 ± 3.20, Tb.N: 2.37 ± 0.50; CDHA/Collagen/rhBMP-2(BMP) group, BV/TV: 14.51 ± 3.12, Tb.N: 2.75 ± 0.65) were significantly higher than those of the control (Blank, BV/TV: 3.25 ± 1.25, Tb.N: 0.57 ± 0.20) group (p < 0.05). Although there was no significant difference between the two experimental groups, the BMP group results were slightly higher than those of the CDHA group (p > 0.05). Moreover, the histological results also supported the micro-CT results. The scaffold of CDHA/collagen seems to be a suitable bio-ceramic carrier loaded rhBMP-2, and appears to enhance new bone formation and bone regeneration in bone defect after implantation.
在本文中，我们主要评估了使用缺钙羟基磷灰石（CDHA）/胶原蛋白基生物陶瓷支架作为骨形态发生蛋白-2（BMP-2）载体修复大鼠颅骨临界尺寸骨缺损时新形成的骨组织。在实时聚合酶链反应分析中，负载重组人骨形态发生蛋白-2（rhBMP-2）的CDHA/胶原蛋白支架组显示骨相关基因表达结果显著增强（p < 0.05）。在体内研究中，显微计算机断层扫描（micro-CT）显示，两个实验组（CDHA/胶原蛋白（CDHA）组，骨体积百分比：14.21±3.20，骨小梁数量：2.37±0.50；CDHA/胶原蛋白/rhBMP-2（BMP）组，骨体积百分比：14.51±3.12，骨小梁数量：2.75±0.65）的主要骨形成参数显著高于对照组（空白组，骨体积百分比：3.25±1.25，骨小梁数量：0.57±0.20）（p < 0.05）。虽然两个实验组之间没有显著差异，但BMP组的结果略高于CDHA组（p > 0.05）。此外，组织学结果也支持了micro-CT结果。CDHA/胶原蛋白支架似乎是一种适合负载rhBMP-2的生物陶瓷载体，并且在植入后似乎能促进骨缺损处的新骨形成和骨再生。
