全基因组鉴定小 G 蛋白 ROPs 及其在茄科家族中的潜在作用。

Genome-wide identification of small G protein ROPs and their potential roles in Solanaceous family.

机构信息

College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, 010019 Hohhot, China; Vegetable Institute, Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences, 010031 Hohhot, China; Laboratory of Phytopathology, Wageningen University and Research, 6708 PB Wageningen, the Netherlands.

College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, 010019 Hohhot, China.

出版信息

Gene. 2020 Aug 30;753:144809. doi: 10.1016/j.gene.2020.144809. Epub 2020 May 26.

DOI:10.1016/j.gene.2020.144809

PMID:32470503

Abstract

Small GTPases function as molecular switches to active or inactive signaling cascades via binding or hydrolyzing GTP. A type of plant specific small GTPases, the ROPs are known to be involved in plant growth, development and immunity. We determined whether ROPs are conserved in Solanaceous species and whether they are involved in plant growth, development and resistance against Phytophthora capsisi. In genome-wide screening, a total of 66 ROPs in six Solanaceous species (SolROPs) were identified, including 16 ROPs in Solanum tuberosum L. (potato), 9 in Solanum lycopersicum L. (tomato), 5 in Solanum melongena L. (eggplant), 9 in Capsicum annuum L. (pepper), 13 in Nicotiana benthamiana Domin and 14 in Nicotiana tabacum L. (tobacco). Phylogenetic analysis revealed that 11 AtROPs and 66 SolROPs fall into five distinct clades (I-V) and hence a novel and systematic gene nomenclature was proposed. In addition, a comprehensive expression analysis was performed by making use of an online database. This revealed that ROP genes are differentially expressed during plant growth and development. Moreover, gene expression of SlROP-II.1 in S. lycopersicum could be significantly induced by P. capsici. Subsequently, SlROP-II.1 and its homologues in N. benthamiana and C. annuum (NbROP-II.1 and CaROP-II.1) were selected for functional analysis using virus-induced gene silencing. Infection assays with P. capsici on silenced plants revealed that SlROP-II.1, NbROP-II.1 and CaROP-II.1 play a role in P. capsici resistance, suggesting conserved function of ROP-II clade across different Solanaceous species. In addition, NbROP-II.1 is also involved in regulating plant growth and development. This study signified the diversity of Solanaceous ROPs and their potential roles in plant growth, development and immunity.

摘要

小分子 GTPases 作为分子开关，通过结合或水解 GTP 来激活或失活信号级联。一种植物特异性的小分子 GTPases，ROP 被认为参与植物的生长、发育和免疫。我们确定 ROP 是否在茄科物种中保守，以及它们是否参与植物的生长、发育和对辣椒疫霉的抗性。在全基因组筛选中，在六个茄科物种（SolROPs）中共鉴定出 66 个 ROP，包括马铃薯（Solanum tuberosum L.）中的 16 个 ROP、番茄（Solanum lycopersicum L.）中的 9 个 ROP、茄子（Solanum melongena L.）中的 5 个 ROP、辣椒（Capsicum annuum L.）中的 9 个 ROP、毕氏烟草（Nicotiana benthamiana Domin）中的 13 个 ROP 和烟草（Nicotiana tabacum L.）中的 14 个 ROP。系统发育分析表明，11 个 AtROPs 和 66 个 SolROPs 分为五个不同的分支（I-V），因此提出了一种新的、系统的基因命名法。此外，通过利用在线数据库进行了全面的表达分析。这表明 ROP 基因在植物的生长和发育过程中表达不同。此外，在番茄（S. lycopersicum）中 SlROP-II.1 的基因表达可以被辣椒疫霉显著诱导。随后，选择 SlROP-II.1 及其在毕氏烟草和辣椒（NbROP-II.1 和 CaROP-II.1）中的同源物进行功能分析，使用病毒诱导的基因沉默。对沉默植物进行辣椒疫霉感染试验表明，SlROP-II.1、NbROP-II.1 和 CaROP-II.1 参与了辣椒疫霉的抗性，表明 ROP-II 分支在不同的茄科物种中具有保守的功能。此外，NbROP-II.1 还参与调节植物的生长和发育。这项研究表明了茄科 ROP 的多样性及其在植物生长、发育和免疫中的潜在作用。