Department of Biotechnology, School of Biosciences, Periyar University, Salem 636011, Tamil Nadu, India.
Department of Chemistry, Siddaganga Institute of Technology, Tumakuru 572103, Karnataka, India.
Environ Toxicol Pharmacol. 2020 Oct;79:103425. doi: 10.1016/j.etap.2020.103425. Epub 2020 May 26.
In this study, we report the antioxidant and antitoxic potential of chemically synthesized 4-oxo-2-phenyl-4H-chromene-7,8-diyl bis((1-amino-2-hydroxypropyl)carbamate) (DHF-BAHPC) compound using in vitro and in vivo assays. The DHF-BAHPC was synthesized by linking 7,8-Dihydroxyflavone (DHF) with two molecules of Fmoc-threonine and characterized by Ultraviolet-visible spectroscopy (UV-vis), Fourier-transform infrared spectroscopy (FT-IR), H NMR, C NMR and Mass spectrometry (MS). In vitro, antioxidant assay results revealed that DHF-BAHPC has a dose-dependent radical scavenging potential towards DPPH, ABTS, FRAP and HO radicals with an IC range of 15.45, 66.27, 25.71, 4.375 μg/mL, respectively. Furthermore DHF-BAHPC treatment significantly altered cadmium (Cd) intoxicated zebrafish embryos by rescuing the developmental changes associated with severe histological and reduced the level of defensive antioxidant activities (SOD, CAT, GPx and GST). The overall results of the present study represented that DHF-BAHPC may be used as a potential drug in redox-based therapeutics.
在这项研究中，我们报告了通过体外和体内实验研究化学合成的 4-氧代-2-苯基-4H-色烯-7,8-二基双((1-氨基-2-羟基丙基)氨基甲酸酯)(DHF-BAHPC)化合物的抗氧化和抗毒性潜力。DHF-BAHPC 通过将 7,8-二羟基黄酮(DHF)与两个 Fmoc-苏氨酸分子连接合成，并通过紫外-可见光谱(UV-vis)、傅里叶变换红外光谱(FT-IR)、H NMR、C NMR 和质谱(MS)进行了表征。在体外，抗氧化测定结果表明，DHF-BAHPC 对 DPPH、ABTS、FRAP 和 HO 自由基具有剂量依赖性的自由基清除能力，IC 范围分别为 15.45、66.27、25.71 和 4.375μg/mL。此外，DHF-BAHPC 处理显著改变了镉(Cd)中毒斑马鱼胚胎，挽救了与严重组织学变化相关的发育变化，并降低了防御性抗氧化活性(SOD、CAT、GPx 和 GST)的水平。本研究的总体结果表明，DHF-BAHPC 可作为基于氧化还原的治疗中的潜在药物。
