Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13/29, 10-718 Olsztyn, Poland.
Regional Veterinary Inspectorate in Gdańsk, Na Stoku 50, 80-958 Gdańsk, Poland.
Toxins (Basel). 2020 May 27;12(6):350. doi: 10.3390/toxins12060350.
The aim of the present study was to determine the effect of zearalenone (ZEN), administered to gilts at doses equivalent to 50%, 100%, and 150% of no-observed-adverse-effect level (NOAEL) values for 14, 28, and 42 days during weaning, on changes in the parameters of the oxidoreductive balance, cytokine secretion, and basal metabolism in ileal Payer's patches. Immunoenzymatic ELISA tests and biochemical methods were used to measure the concentrations of interleukin 1α, interleukin 1β, interleukin 12/23p40, interleukin 2, interferon γ, interleukin 4, interleukin 6, interleukin 8, tumor necrosis factor α, interleukin 10, transforming growth factor β, malondialdehyde, sulfhydryl groups, fructose, glucose, and proline, as well as the activity of peroxidase, superoxide dismutase and catalase. The study demonstrated that ZEN doses corresponding to 50%, 100%, and 150% of NOAEL values, i.e., 5 µg, 10 µg, and 15 µg ZEN/kg BW, respectively, have proinflammatory properties, exacerbate oxidative stress responses, and disrupt basal metabolism in ileal Payer's patches in gilts.
本研究旨在确定在断奶期间以相当于无观察到不良作用水平（NOAEL）值的 50%、100%和 150%的剂量（分别为 5µg、10µg 和 15µg ZEN/kgBW）给后备母猪连续 14、28 和 42 天喂食玉米赤霉烯酮（ZEN）对回肠派尔氏斑氧化还原平衡、细胞因子分泌和基础代谢参数变化的影响。采用免疫酶联 ELISA 试验和生化方法测量白细胞介素 1α、白细胞介素 1β、白细胞介素 12/23p40、白细胞介素 2、干扰素 γ、白细胞介素 4、白细胞介素 6、白细胞介素 8、肿瘤坏死因子 α、白细胞介素 10、转化生长因子 β、丙二醛、巯基、果糖、葡萄糖和脯氨酸的浓度，以及过氧化物酶、超氧化物歧化酶和过氧化氢酶的活性。研究表明，相当于 NOAEL 值的 50%、100%和 150%的 ZEN 剂量，即 5µg、10µg 和 15µg ZEN/kgBW，分别具有促炎特性，加重氧化应激反应，并破坏后备母猪回肠派尔氏斑的基础代谢。
