Department of Environment, Faculty of Forestry and Environment, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
BMC Complement Med Ther. 2020 May 29;20(1):160. doi: 10.1186/s12906-020-02960-1.
To investigate the protective effects of Tualang honey against the toxicity effects induced by cadmium (Cd) on the ovary.
A total of 32 female Sprague Dawley rats were taken and randomly divided into four groups (n = 8). Throughout the experimental period of 6 weeks, negative control-NC (vehicle deionized water), positive control-CD (Cd at 5 mg/kg), Tualang honey followed by Cd exposure-TH (Tualang honey at 200 mg/kg and Cd at 5 mg/kg) and Tualang honey control-THC (Tualang honey at 200 mg/kg) groups, were administered orally on a daily basis.
Rats exposed to Cd were significantly higher in ovarian weight, number of antral and atretic follicles as compared to the NC group. The disruptive effects of Cd on ovarian follicles were associated with a disruption in gonadotropin hormones and decreases in follicular stimulating hormone (FSH) and luteinizing hormone (LH). Moreover, a significant formation of oxidative stress in ovarian Cd-exposed rats has been proven by increasing the level of lipid peroxidation products (malondialdehyde) and decreasing the levels of enzymatic antioxidant (catalase). Interestingly, a daily supplementation of high antioxidant agents such as Tualang honey in these animals, caused significant improvements in the histological changes. Additionally, less atretic follicles were observed, restoring the normal level of LH and FSH (P < 0.001), and normalizing the ovarian malondialdehyde (P < 0.05) and catalase levels in comparison with CD group (P < 0.05).
Tualang honey has protective effects against Cd-induced ovarian toxicity by reducing morphological abnormalities, restoring the normal levels of gonadotropin hormones and stabilizing equilibrium levels of lipid peroxidation and antioxidant enzyme in ovaries of rats.
研究目的是探讨塔兰蜂蜜（Tualang honey）对镉（Cd）诱导的卵巢毒性的保护作用。
将 32 只雌性 Sprague Dawley 大鼠随机分为四组（每组 8 只）。在 6 周的实验期间，阴性对照组（赋形剂去离子水）、阳性对照组（Cd 5mg/kg）、塔兰蜂蜜联合 Cd 暴露组（塔兰蜂蜜 200mg/kg 和 Cd 5mg/kg）和塔兰蜂蜜对照组（塔兰蜂蜜 200mg/kg）分别每日灌胃给药。
与 NC 组相比，暴露于 Cd 的大鼠卵巢重量、腔前卵泡和闭锁卵泡数量显著增加。Cd 对卵巢卵泡的破坏作用与促性腺激素的破坏和卵泡刺激素（FSH）和黄体生成素（LH）的减少有关。此外，Cd 暴露的卵巢大鼠中氧化应激的形成显著增加，表现为脂质过氧化产物（丙二醛）水平升高，酶抗氧化剂（过氧化氢酶）水平降低。有趣的是，这些动物每天补充高抗氧化剂，如塔兰蜂蜜，可显著改善组织学变化。此外，观察到较少的闭锁卵泡，LH 和 FSH 水平恢复正常（P<0.001），并且与 CD 组相比，卵巢丙二醛（P<0.05）和过氧化氢酶水平恢复正常（P<0.05）。
塔兰蜂蜜对 Cd 诱导的卵巢毒性具有保护作用，可减少形态学异常，恢复促性腺激素水平正常，并稳定大鼠卵巢中脂质过氧化和抗氧化酶的平衡水平。
