Zaid Siti Sarah Mohamad, Othman Shatrah, Kassim Normadiah M
Department of Anatomy, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
BMC Complement Altern Med. 2014 Dec 17;14:509. doi: 10.1186/1472-6882-14-509.
To investigate the potential protective effects of Tualang honey against the toxicity effects induced by Bisphenol A (BPA) on pubertal development of ovaries.
This study was conducted on pre-pubertal female Sprague Dawley rats. Animals were divided into four groups (n = 8 in each group). Group I was administered with vehicle 0.2 ml of corn oil (Sigma-Aldrich, USA) using oral gavage daily for six weeks; these animals served as negative control (CO group), Group II was administered with BPA suspended in corn oil at 10 mg/kg body weight and served as positive control (PC group), Group III was administered with 200 mg/kg body weight of Tualang honey 30 min before the administration of BPA at 10 mg/kg (TH group) while Group IV was administered with 200 mg/kg body weight of Tualang honey 30 min before the administration of corn oil (THC group). Body weight of all animals were monitored weekly.
The BPA-exposed animals exhibited disruption of their estrus cycle, while those animals treated with BPA together with Tualang honey, exhibited an improvement in percentage of normal estrous cycle. Their ovaries had lower numbers of atretic follicles compared to the PC group but higher than the CO group.
Tualang honey has a potential role in reducing BPA-induced ovarian toxicity by reducing the morphological abnormalities of the ovarian follicles and improving the normal estrous cycle.
探讨特浪蜂蜜对双酚A（BPA）诱导的青春期卵巢毒性作用的潜在保护作用。
本研究以青春期前雌性斯普拉格-道利大鼠为对象。动物分为四组（每组n = 8）。第一组每天经口灌胃给予0.2 ml玉米油（美国西格玛奥德里奇公司），持续六周；这些动物作为阴性对照（玉米油组），第二组给予悬浮于玉米油中的双酚A，剂量为10 mg/kg体重，作为阳性对照（双酚A组），第三组在给予10 mg/kg双酚A前30分钟给予200 mg/kg体重的特浪蜂蜜（特浪蜂蜜 + 双酚A组），而第四组在给予玉米油前30分钟给予200 mg/kg体重的特浪蜂蜜（特浪蜂蜜 + 玉米油组）。每周监测所有动物的体重。
暴露于双酚A的动物出现发情周期紊乱，而同时给予双酚A和特浪蜂蜜治疗的动物，正常发情周期百分比有所改善。与双酚A组相比，它们的卵巢闭锁卵泡数量较少，但高于玉米油组。
特浪蜂蜜通过减少卵巢卵泡的形态异常和改善正常发情周期，在减轻双酚A诱导的卵巢毒性方面具有潜在作用。
