Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China.
Fish Physiol Biochem. 2020 Aug;46(4):1507-1518. doi: 10.1007/s10695-020-00809-5. Epub 2020 May 29.
The blood acts as a transfer channel for a variety of factors in the whole body. The ricefield eel (Monopterus albus) is a protogynous hermaphrodite vertebrate. Until now, no research has reported an analysis of the blood transcriptome during the process of sexual development in the ricefield eel. In this study, the transcriptome sequencing of blood samples from male and female ricefield eels was completed with a total of 34.70 Gb clean data. The clean data of each sample all reached 5.23 GB, and the percent of the Q30 basic group was 88.62% and above. A total of 106,369 unigenes were obtained after assembly, including 13,296 unigenes with a length of more than 1 kb. Further functional annotation analysis showed that there are 28,522 unigenes that can be annotated. The annotations of genes with differential expression revealed that there were 563 genes with significant differential expression in the blood of male and female ricefield eels, including 91 upregulated genes and 472 downregulated genes. Among which, 14 genes may be closely related to sex differentiation, the qPCR was used to confirmed the expression pattern of those genes and result shown that 11 genes were downregulated and 3 genes were upregulated, consistent with the results of our RNA-Seq analysis. This blood transcript dataset will open future research avenues on ricefield eel sex development and differentiation.
血液作为全身各种因素的传输通道。 稻田鳗（Monopterus albus）是一种雌雄同体的脊椎动物。 到目前为止，还没有研究报道过对稻田鳗性发育过程中的血液转录组进行分析。 在这项研究中，我们完成了雌雄稻田鳗血液样本的转录组测序，共获得 34.70Gb 的清洁数据。 每个样本的清洁数据均达到 5.23GB，Q30 碱基组的百分比在 88.62%以上。 组装后共获得 106369 条 unigene，其中长度大于 1kb 的 unigene 有 13296 条。 进一步的功能注释分析表明，有 28522 条 unigene 可以被注释。 差异表达基因的注释显示，雌雄稻田鳗血液中有 563 个基因存在显著差异表达，其中上调基因 91 个，下调基因 472 个。 其中，有 14 个基因可能与性别分化密切相关，我们使用 qPCR 验证了这些基因的表达模式，结果显示 11 个基因下调，3 个基因上调，与 RNA-Seq 分析结果一致。 这个血液转录组数据集将为未来研究稻田鳗的性发育和分化开辟新的途径。
