Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou, 515063, China; Marine Biology Institute, Shantou University, Shantou, 515063, China; STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou, 515063, China.
Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou, 515063, China; Marine Biology Institute, Shantou University, Shantou, 515063, China; STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou, 515063, China.
Fish Shellfish Immunol. 2020 Aug;103:430-437. doi: 10.1016/j.fsi.2020.05.069. Epub 2020 May 27.
Vibrio parahaemolyticus (V. parahaemolyticus) is a common pathogen for marine crustacean, which causes severe illnesses in aquatic animals. Therefore, it is meaningful to explore the mechanism during V. parahaemolyticus infection. In this study, to investigate the immune responses of mud crab Scylla paramamosain (S. paramamosain) to V. parahaemolyticus, we established the metabolic and transcriptional profiles of mud crab hemocytes challenged with V. parahaemolyticus. The results indicated that V. parahaemolyticus infection could induce a series of metabolism alterations at both metabolome and transcriptome levels, including biosynthesis of amino acids and Aminoacyl-tRNA, Purine and pyrimidine metabolism, TCA cycle and glutamine metabolism. In this context, through the integration of metabolomics and transcriptomics, our study provided a more comprehensive understanding of the biological process in mud crab against pathogen infection.
副溶血弧菌（Vibrio parahaemolyticus）是一种常见的海洋甲壳类动物病原体，可引起水生动物的严重疾病。因此，探索副溶血弧菌感染过程中的机制具有重要意义。在这项研究中，为了研究泥蟹（Scylla paramamosain）对副溶血弧菌的免疫反应，我们建立了泥蟹血细胞在受到副溶血弧菌刺激时的代谢和转录谱。结果表明，副溶血弧菌感染可在代谢组和转录组水平上诱导一系列代谢改变，包括氨基酸和氨酰-tRNA 的生物合成、嘌呤和嘧啶代谢、三羧酸循环和谷氨酰胺代谢。在这种情况下，通过代谢组学和转录组学的整合，我们的研究提供了对泥蟹抵御病原体感染的生物学过程的更全面的理解。
