Jiang Hongbo, Bao Jie, Xing Yuenan, Cao Gangnan, Li Xiaodong, Chen Qijun
Aquaculture Department, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, China.
Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Shenyang Agricultural University, Shenyang, China.
Front Microbiol. 2022 Sep 23;13:990737. doi: 10.3389/fmicb.2022.990737. eCollection 2022.
Milky disease caused by fungus has significantly harmed the Chinese mitten crab aquaculture industry. However, the effect of infection on the metabolism and intestinal flora of the crab remains unclear. In this study, we aimed to explore the changes in the metabolism and intestinal flora after 48 h of infection with , using metabolomic and metagenomic analyses. Metabolomic analysis results revealed 420 significantly different metabolites between the infected and control groups, and these metabolites were enriched in 58 metabolic pathways. infection decreased the levels of metabolites related to amino acid biosynthesis, the tricarboxylic acid cycle, as well as lysine, histidine, linolenic, arachidonic, and linoleic acid metabolism. These results indicated that infection significantly affected the energy metabolism, growth, and immunity of . The results of metagenomic analysis showed that the anaerobes and ascomycetes populations significantly increased and decreased, respectively, after infection. These changes in intestinal flora significantly upregulated metabolic and synthetic pathways while downregulating immunity-related pathways. The results of integrated metabolomic and metagenomic analyses showed that 55 differentially expressed genes and 28 operational taxonomic units were correlated with 420 differential metabolites. Thus, the intestinal flora changes caused by infection also affected the metabolites. This study provides novel insights into the metabolic-and intestinal microflora-based effects of infection in , as well as a theoretical basis for the interaction between fungi and crustaceans.
由真菌引起的乳白病已对中华绒螯蟹养殖业造成了严重危害。然而，感染对蟹的新陈代谢和肠道菌群的影响仍不清楚。在本研究中，我们旨在通过代谢组学和宏基因组学分析，探索感染[具体真菌名称未给出]48小时后蟹的新陈代谢和肠道菌群的变化。代谢组学分析结果显示，感染组和对照组之间有420种显著不同的代谢物，这些代谢物富集在58条代谢途径中。[具体真菌名称未给出]感染降低了与氨基酸生物合成、三羧酸循环以及赖氨酸、组氨酸、亚麻酸、花生四烯酸和亚油酸代谢相关的代谢物水平。这些结果表明，[具体真菌名称未给出]感染显著影响了蟹的能量代谢、生长和免疫。宏基因组学分析结果表明，感染[具体真菌名称未给出]后，厌氧菌和子囊菌种群分别显著增加和减少。肠道菌群的这些变化显著上调了代谢和合成途径，同时下调了免疫相关途径。代谢组学和宏基因组学综合分析结果表明，55个差异表达基因和28个操作分类单元与420种差异代谢物相关。因此，[具体真菌名称未给出]感染引起的肠道菌群变化也影响了代谢物。本研究为[具体真菌名称未给出]感染对蟹的基于代谢和肠道微生物群的影响提供了新的见解，也为真菌与甲壳类动物之间的相互作用提供了理论基础。
