TLR4 基因多态性与重症肠道病毒 71 感染的相关性。
Correlation between TLR4 gene polymorphism and severe enterovirus 71 infection.
机构信息
Department of Pediatrics, Qingdao Women and Children's Hospital, Qingdao, 266000, China; Department of Pediatrics, The Affiliated Hospital of Qingdao University, Qingdao, 266000, China.
Department of Pediatrics, Qingdao Women and Children's Hospital, Qingdao, 266000, China.
出版信息
J Infect Chemother. 2020 Oct;26(10):1015-1020. doi: 10.1016/j.jiac.2020.05.004. Epub 2020 May 28.
OBJECTIVE
Enterovirus 71 (EV71) infection resulted in high mortality and disability in children. Immune response deficiency and cytokine genetic predispositions were associated with the severity of EV71 infection. We aim to evaluate the association between TLR4 gene polymorphisms and severity of EV71 infection in Chinese children.
METHODS
TLR4 gene polymorphisms were detected through improved multiplex ligation detection reaction technology. TLR4 expression was measured by Real-Time reverse transcriptase PCR and flow cytometry. The plasma levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were analyzed by enzyme-linked immunosorbent assay.
RESULTS
The frequency of rs10759932CC genotype and the C allele was significantly higher in cases with severe EV71 infection than those with mild infection. The levels of TLR4, serum TNF-α and IL-6 in cases with rs10759932CC genotype were also significantly elevated when compared to those with TT genotypes.
CONCLUSIONS
The rs10759932 polymorphism in TLR4 was associated with the severity of EV71 infection. The C allele of rs10759932 may be one of the risk factors of severe EV71 infection in Chinese children.
目的
肠道病毒 71 型（EV71）感染可导致儿童高死亡率和残疾。免疫反应缺陷和细胞因子遗传易感性与 EV71 感染的严重程度有关。本研究旨在评估 TLR4 基因多态性与中国儿童 EV71 感染严重程度的关系。
方法
采用改良多重连接酶检测反应技术检测 TLR4 基因多态性。通过实时逆转录聚合酶链反应和流式细胞术检测 TLR4 表达。采用酶联免疫吸附试验分析肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的血浆水平。
结果
与轻症感染相比，重症 EV71 感染患者 TLR4 基因 rs10759932CC 基因型和 C 等位基因的频率显著升高。与 TT 基因型相比，rs10759932CC 基因型患者的 TLR4 水平、血清 TNF-α 和 IL-6 水平也明显升高。
结论
TLR4 基因 rs10759932 多态性与 EV71 感染的严重程度有关。rs10759932 的 C 等位基因可能是中国儿童重症 EV71 感染的危险因素之一。
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