白细胞介素-10 基因-592 多态性与中国儿童肠道病毒 71 感染后白细胞介素-10 表达和严重程度相关。
IL-10-592 polymorphism is associated with IL-10 expression and severity of enterovirus 71 infection in chinese children.
机构信息
Neonatal Departmant, The Affiliated Women & Children Hospital of Qingdao University, Qingdao, 266000, China; Pediatric Department, The Affiliated Hospital of Qingdao University, Qingdao, 266000, China.
Neonatal Departmant, The Affiliated Women & Children Hospital of Qingdao University, Qingdao, 266000, China.
出版信息
J Clin Virol. 2017 Oct;95:42-46. doi: 10.1016/j.jcv.2017.08.005. Epub 2017 Aug 19.
BACKGROUND
Enterovirus 71 (EV71) infection results in some severe complications with high mortality and disability in Hand, Foot and Mouth Disease (HFMD) in children. Recent studies have shown that cytokine genetic predispositions have associations with both the development of EV71 infection and severity of HFMD.
OBJECTIVE
This study was designed to investigate whether the IL-10-592 polymorphism is associated with IL-10 levels and disease severity in Chinese children with EV71 infection.
STUDY DESIGN
In patients selected, there were 378 cases with EV71 infection (including 291 mild cases, 70 severe cases and 17 critical cases), as well as 406 health controls. EV71 in serum was tested by RT-PCR, and IL-10-592 genotype was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis techniques.
RESULT
The IL-10-592C allele was observed with higher frequency in patients with critical EV71 infection (70.59%) compared with severe EV71 infection (41.43%, P<0.01), mild EV71 infection (43.81%, P<0.01) and healthy children (44.46%, P<0.01). The blood IL-10 levels of critical cases were significantly higher than severe cases, mild cases, and healthy children. Among all of the four groups, IL-10 levels in patients with genotype AA were significantly lower than those with genotypes AC+CC (t=4.86, P<0.05; t=2.30, P<0.05; t=3.44, P<0.05; t=5.58, P<0.05).
CONCLUSION
IL-10-592C allele is associated with IL-10 expressions and the severity of EV71 infection in Chinese patients.
背景
肠道病毒 71 型（EV71）感染可导致手足口病（HFMD）患儿出现一些严重并发症，死亡率和致残率较高。最近的研究表明，细胞因子遗传倾向与 EV71 感染的发展和 HFMD 的严重程度有关。
目的
本研究旨在探讨白细胞介素 10（IL-10）-592 多态性是否与中国 EV71 感染患儿的 IL-10 水平和疾病严重程度有关。
研究设计
在入选的患者中，有 378 例 EV71 感染患者（包括 291 例轻症、70 例重症和 17 例危重症）和 406 例健康对照。采用 RT-PCR 检测血清中的 EV71，聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析技术检测 IL-10-592 基因型。
结果
危重症 EV71 感染患者的 IL-10-592C 等位基因频率（70.59%）明显高于重症 EV71 感染（41.43%，P<0.01）、轻症 EV71 感染（43.81%，P<0.01）和健康儿童（44.46%，P<0.01）。危重症患者的血 IL-10 水平明显高于重症、轻症和健康儿童。在这四组患者中，基因型 AA 的患者的 IL-10 水平明显低于基因型 AC+CC 的患者（t=4.86，P<0.05；t=2.30，P<0.05；t=3.44，P<0.05；t=5.58，P<0.05）。
结论
IL-10-592C 等位基因与中国 EV71 感染患者的 IL-10 表达和感染严重程度有关。
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