Zhao Na, Li Jing, Chen Zhen-Zhen, Chu Zhu-Fei, Chen Zong-Bo
Department of Neonatology, Women & Children's Hospital Affiliated to Qingdao University, Qingdao, Shandong 266000, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2019 Aug;21(8):789-795. doi: 10.7499/j.issn.1008-8830.2019.08.010.
To study the association of interleukin-10 (IL-10) -1082A/G, -819C/T, and -592C/A polymorphisms with IL-10 level and the severity of enterovirus 71 (EV71) infection in children.
A total of 137 children with hand-foot-mouth disease due to EV71 infection were enrolled as EV71 infection group, which was further divided into mild group with 91 children and severe group with 46 children, and 122 healthy children who underwent physical examination were enrolled as healthy control group. Related clinical data were collected. ELISA was used to measure the serum level of IL-10, and polymerase chain reaction-restriction fragment length polymorphism was used to analyze IL-10 -1082A/G, -819C/T and -592C/A polymorphisms.
Compared with the healthy control group, the children with EV71 infection had significantly higher frequency of -1082 AA genotype and A allele (P<0.05). Among the children with EV71 infection, the severe group had significantly higher frequency of -1082 AA genotype and A allele than the mild group (P<0.05), while there was no significant difference in the distribution of IL-10 -819C/T and IL-10 -592C/A polymorphisms between the two groups (P>0.05). The severe group had a significantly higher serum level of IL-10 than the mild group and the healthy control group. IL-10 -1082 AA genotype, -819 TT genotype, and -592 AA genotype were associated with the low expression of IL-10 (P<0.05). As for haplotype, the EV71 infection group had a significantly lower frequency of GCC haplotype than the healthy control group (P<0.05). In the severe group, the children with ATA haplotype had a significantly lower IL-10 level than those with other haplotypes, and the children with GCC haplotype had a significantly higher IL-10 level than those with other haplotypes (P<0.05). There was no significant difference in IL-10 level between children with different haplotypes in the mild group and the healthy control group (P>0.05).
IL-10 gene polymorphisms are associated with IL-10 expression and the severity of EV71 infection in children.
研究白细胞介素-10（IL-10）-1082A/G、-819C/T和-592C/A基因多态性与儿童肠道病毒71型（EV71）感染时IL-10水平及病情严重程度的关系。
选取137例EV71感染所致手足口病患儿作为EV71感染组，进一步分为轻症组91例和重症组46例；选取122例进行健康体检的儿童作为健康对照组。收集相关临床资料。采用酶联免疫吸附测定（ELISA）法检测血清IL-10水平，采用聚合酶链反应-限制性片段长度多态性方法分析IL-10 -1082A/G、-819C/T和-592C/A基因多态性。
与健康对照组相比，EV71感染患儿-1082 AA基因型和A等位基因频率显著升高（P<0.05）。在EV71感染患儿中，重症组-1082 AA基因型和A等位基因频率显著高于轻症组（P<0.05），而两组间IL-10 -819C/T和IL-10 -592C/A基因多态性分布差异无统计学意义（P>0.05）。重症组血清IL-10水平显著高于轻症组和健康对照组。IL-10 -1082 AA基因型、-819 TT基因型和-592 AA基因型与IL-10低表达有关（P<0.05）。单倍型方面，EV71感染组GCC单倍型频率显著低于健康对照组（P<0.05）。在重症组中，ATA单倍型患儿的IL-10水平显著低于其他单倍型患儿，GCC单倍型患儿的IL-10水平显著高于其他单倍型患儿（P<0.05）。轻症组和健康对照组不同单倍型患儿的IL-10水平差异无统计学意义（P>0.05）。
IL-10基因多态性与儿童IL-10表达及EV71感染的严重程度有关。
