Chemistry Department, Faculty of Science, Kafrelsheikh University, Kafrelsheikh, 33516, Egypt.
Umm Al-Qura University, University College in Al-Jamoum, Chemistry Department, 21955 Makkah, Saudi Arabia.
Int J Biol Macromol. 2020 Oct 1;160:1230-1241. doi: 10.1016/j.ijbiomac.2020.05.224. Epub 2020 May 29.
This study aimed to present a new heart of P. dactylifera (HP) extract loaded chitosan nanoparticles and estimate its anticancer, antimicrobial, antioxidant activity and free radical scavenger effect (in vitro). This nano-supplement may prevent doxorubicin cardiotoxicity and nephrotoxicity in rat model. The HP extract was loaded on chitosan nanoparticles producing HP-ChNPs, then characterized. The antioxidant properties of the HP-ChNPs was assessed in vitro. The antibacterial activity against three-gram positive bacteria and two gram-negative bacteria were done. The in vitro studies of cytotoxicity against MCF7, CaCo3, and Hela cell lines were also evaluated. Then, the protective effect of the HP-ChNPs (2 mg/kg, IP) was evaluated against doxorubicin induce organ toxicity in a rat model. The in vitro studies revealed the antibacterial, anticancer and antioxidant activities of the HP-ChNPs. The in vivo study demonstrates reduction of heart and kidney apoptosis with increased programmed cell death protein-1 (PD-1); as the major anticancer drug (doxorubicin) pathway is to release free radicals with decreased PD-1 levels and induction of apoptosis. In conclusion, the HP-ChNPs, in a very small dose, might be a promising supplement to avoid doxorubicin toxicity with improvment the antioxidant enzymes without affecting its anticancer activity.
本研究旨在介绍一种新型的乳清蛋白（HP）提取物负载壳聚糖纳米粒子，并评估其体外抗癌、抗菌、抗氧化活性和自由基清除作用。这种纳米补充剂可能有助于预防 doxorubicin 对大鼠模型的心脏毒性和肾毒性。将 HP 提取物负载到壳聚糖纳米粒子上，生成 HP-ChNPs，然后对其进行表征。体外评估 HP-ChNPs 的抗氧化特性。对三种革兰氏阳性菌和两种革兰氏阴性菌进行了抗菌活性测试。还评估了对 MCF7、CaCo3 和 Hela 细胞系的体外细胞毒性研究。然后，评估了 HP-ChNPs（2mg/kg，IP）对 doxorubicin 诱导的大鼠模型器官毒性的保护作用。体外研究表明 HP-ChNPs 具有抗菌、抗癌和抗氧化活性。体内研究表明，通过增加程序性细胞死亡蛋白-1（PD-1），减少心脏和肾脏凋亡；因为主要的抗癌药物（doxorubicin）途径是释放自由基，降低 PD-1 水平并诱导细胞凋亡。总之，在非常小的剂量下，HP-ChNPs 可能是一种有前途的补充剂，可以避免 doxorubicin 毒性，同时提高抗氧化酶水平，而不影响其抗癌活性。
