Bel'skaya Lyudmila V, Sarf Elena A, Shalygin Sergey P, Postnova Tatyana V, Kosenok Victor K
Biochemistry Research Laboratory, Omsk State Pedagogical University, 14, Tukhachevsky Str, Omsk, 644043, Russia.
Department of Chemistry, Omsk State Medical University, 12, Lenina Str, Omsk, 644099, Russia.
J Oral Biosci. 2020 Jun;62(2):212-221. doi: 10.1016/j.job.2020.05.002. Epub 2020 May 29.
The purpose of the pilot study was to determine the potential diagnostic capabilities for the analysis of oxygen-containing salivary volatile organic compounds (VOCs) in stomach and colorectal cancer.
Saliva samples of 11 patients with stomach cancer, 18 patients with colorectal cancer, and 16 healthy volunteers were analyzed through capillary gas chromatography. The levels of lipid peroxidation products and catalase activity were determined in all samples. To assess saliva diagnostic potential, we constructed a Classification and Regression Tree (CART).
It was shown that the use of a combination of saliva VOCs (acetaldehyde, acetone, propanol-2, and ethanol) allowed classification into Cancer/Control groups with a sensitivity and specificity of 95.7 and 90.9%, respectively. To clarify the location of the tumor, it was necessary to add a methanol level; in this case, the sensitivity for detecting stomach and colorectal cancer was 80.0% and 92.3%, respectively, while the specificity in both cases was 100%. When the lipid peroxidation product content was added to the VOC indicators, they were selected as the main factors for constructing the decision tree. For classification into Cancer/Control groups, only the triene conjugate and Schiff base content in saliva was sufficient. The combination of VOCs in saliva and lipid peroxidation indices improved the sensitivity and specificity for classification to 100%.
Preliminary data were obtained on the sensitivity and specificity of the diagnosis of stomach and colorectal cancer, which confirmed the promise of further studies on saliva VOCs for the purpose of clinical laboratory diagnostics.
本初步研究的目的是确定分析胃癌和结直肠癌患者唾液中含氧挥发性有机化合物（VOCs）的潜在诊断能力。
通过毛细管气相色谱法分析了11例胃癌患者、18例结直肠癌患者和16名健康志愿者的唾液样本。测定了所有样本中脂质过氧化产物的水平和过氧化氢酶活性。为了评估唾液的诊断潜力，我们构建了分类回归树（CART）。
结果表明，联合使用唾液VOCs（乙醛、丙酮、2-丙醇和乙醇）可将样本分为癌症/对照组，灵敏度和特异度分别为95.7%和90.9%。为了明确肿瘤的位置，有必要加入甲醇水平；在这种情况下，检测胃癌和结直肠癌的灵敏度分别为80.0%和92.3%，而两种情况下的特异度均为100%。当将脂质过氧化产物含量添加到VOC指标中时，它们被选为构建决策树的主要因素。对于癌症/对照组的分类，仅唾液中的三烯共轭物和席夫碱含量就足够了。唾液中的VOCs与脂质过氧化指标的组合将分类的灵敏度和特异度提高到了100%。
获得了关于胃癌和结直肠癌诊断灵敏度和特异度的初步数据，这证实了进一步研究唾液VOCs用于临床实验室诊断的前景。
