一种用于次氯酸盐和铜（II）离子检测及生物成像应用的新型双功能芘基开启型荧光探针。
A novel dual functional pyrene-based turn-on fluorescent probe for hypochlorite and copper (II) ion detection and bioimaging applications.
作者信息
Yin Jie, Wang Zhonglong, Zhao Fei, Yang Haiyan, Li Mingxin, Yang Yiqin
机构信息
Co-innovation Center of Efficient Processing and Utilization of Forest Resources, College of Light Industry and Food, College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China.
Co-innovation Center of Efficient Processing and Utilization of Forest Resources, College of Light Industry and Food, College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Oct 5;239:118470. doi: 10.1016/j.saa.2020.118470. Epub 2020 May 12.
A novel dual-function fluorescent probe PPH was conveniently synthesized by linking pyrazinehydrazide unit and pyrenyl fluorophore. The present probe PPH could be used for simultaneous detection of ClO and Cu through a fluorescence turn-on response. The probe PPH displayed excellent selectivity and sensitivity towards ClO and Cu over other anions and metal ions. The detection limits of PPH for ClO and Cu were calculated to be as low as 4.02 nM and 157 nM, respectively. The probe PPH had good pH stability and was highly responsive to ClO and Cu in a wide pH range. Furthermore, this probe could be readily applied to visualize ClO and Cu on the test paper. Additionally, this probe was successfully utilized to the fluorescence imaging of ClO and Cu in HeLa cells.
通过连接吡嗪酰肼单元和芘基荧光团，简便地合成了一种新型双功能荧光探针PPH。本探针PPH可通过荧光开启响应同时检测ClO和Cu。与其他阴离子和金属离子相比，探针PPH对ClO和Cu表现出优异的选择性和灵敏度。PPH对ClO和Cu的检测限经计算分别低至4.02 nM和157 nM。探针PPH具有良好的pH稳定性，在较宽的pH范围内对ClO和Cu有高度响应。此外，该探针可轻松应用于在试纸上可视化ClO和Cu。此外，该探针成功用于HeLa细胞中ClO和Cu的荧光成像。
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