Department of Biochemistry, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
Department of Food Science and Technology, College of Agriculture, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
Biomarkers. 2020 Jul;25(5):384-390. doi: 10.1080/1354750X.2020.1754912. Epub 2020 Jun 1.
The aim of this study was to evaluate the methylation status of TNF-α and SOCS3 promoters in patients with BD and compare them with a healthy group. This was a case-control study, in which 47 subjects with BD and 61 individuals as the control participated. Blood samples were collected from all the participants. Then, PBMCs were isolated using the Ficoll method and methylation of considered sites was investigated using the qMS-PCR technique after DNA extraction by the rapid genomic DNA extraction method and its analysis with Nano-drop. The methylation and expression of TNF-α showed that the methylation level significantly declined in the patient in comparison with the healthy ( < 0.05). Moreover, the results on the mean expression showed that it significantly increased in the patient group, as compared with the healthy group ( < 0.05). In addition, the expression of the SOCS3 gene was not significantly different between the patients and healthy subjects while the level of SOCS3 methylation was significantly higher in the patient group than that in the healthy group ( < 0.05). The present study revealed that the gene expression of TNF-alpha increased in BD patients, suggesting that TNF-alpha likely has a role in the pathogenesis of BD.
本研究旨在评估 TNF-α 和 SOCS3 启动子在 BD 患者中的甲基化状态，并将其与健康组进行比较。这是一项病例对照研究，共纳入 47 例 BD 患者和 61 名健康对照者。所有参与者均采集血样。然后采用 Ficoll 法分离 PBMCs，采用 qMS-PCR 技术在快速基因组 DNA 提取法提取 DNA 并经 Nano-drop 分析后，检测考虑位点的甲基化情况。TNF-α 的甲基化和表达显示，与健康组相比，患者组的甲基化水平显著降低（<0.05）。此外，与健康组相比，患者组的平均表达显著增加（<0.05）。此外，SOCS3 基因的表达在患者和健康受试者之间没有显著差异，而 SOCS3 甲基化水平在患者组显著高于健康组（<0.05）。本研究表明，BD 患者中 TNF-α 基因表达增加，提示 TNF-α 可能在 BD 的发病机制中起作用。
