Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
Poult Sci. 2020 Jun;99(6):2861-2872. doi: 10.1016/j.psj.2019.12.067. Epub 2020 Mar 19.
Large ovarian follicles are primary characteristics of oviparous species. The development of such follicles is crucially governed by strict intrinsic complex regulation. Many aspects of the genetic basis of this regulation remain obscure. To identify the dominant genes controlling follicular development in the chicken, growing follicles (400-1,600 μm in diameter) were selected for RNA sequencing and bioinformatics analysis. Comparing the 400-μm follicles with 800-μm follicles identified a total of 3,627 differentially expressed genes (1,792 upregulated and 1,835 downregulated genes). Comparing the 400-μm follicles with 1,600-μm follicles revealed 9,650 differentially expressed genes (including 4,848 upregulated and 4,802 downregulated genes). Comparing 800-μm with 1,600-μm follicles revealed a total of 6,779 differentially expressed genes (3,427 upregulated and 3,352 downregulated genes). Transcriptome analysis revealed that genes related to the extracellular matrix-receptor interactions, steroid biosynthesis, cell adhesion, and phagosomes displayed remarkable differential expressions. Relative to 400-μm follicles, collagen content, production of steroid hormones, cell adhesion, and phagocytic factors were significantly increased in the 1,600-μm follicles. This study identifies the dominant genes involved in the promotion of follicular development in oviparous vertebrates and represents the extraordinary gene regulation pattern related to development of the growing follicles in poultry.
大卵泡是卵生动物的主要特征。这些卵泡的发育受到严格的内在复杂调节的严格控制。该调节的遗传基础的许多方面仍然不清楚。为了鉴定控制鸡卵泡发育的优势基因，选择生长卵泡（直径 400-1600μm）进行 RNA 测序和生物信息学分析。将 400μm 卵泡与 800μm 卵泡进行比较，共鉴定出 3627 个差异表达基因（1792 个上调和 1835 个下调基因）。将 400μm 卵泡与 1600μm 卵泡进行比较，发现 9650 个差异表达基因（包括 4848 个上调和 4802 个下调基因）。将 800μm 卵泡与 1600μm 卵泡进行比较，共发现 6779 个差异表达基因（3427 个上调和 3352 个下调基因）。转录组分析显示，与细胞外基质-受体相互作用、类固醇生物合成、细胞黏附和吞噬体相关的基因表达差异显著。与 400μm 卵泡相比，1600μm 卵泡中的胶原蛋白含量、类固醇激素的产生、细胞黏附和吞噬因子明显增加。本研究鉴定了参与卵生脊椎动物卵泡发育的优势基因，代表了与家禽生长卵泡发育相关的非凡基因调控模式。
