College of Animal Science and Technology, Guangxi University, Nanning, 530004, China.
Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Guangxi University, Nanning, 530004, China.
BMC Genomics. 2023 Aug 3;24(1):433. doi: 10.1186/s12864-023-09521-z.
The avian eggshell is formed in the uterus. Changes in uterine function may have a significant effect on eggshell quality. To identify the vital genes impacting uterine functional maintenance in the chicken, uteri in three different periods (22W, 31W, 51W) were selected for RNA sequencing and bioinformatics analysis. In our study, 520, 706 and 736 differentially expressed genes (DEGs) were respectively detected in the W31 vs W22 group, W51 vs W31 group and W51 vs W22 group. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated DEGs were enriched in the extracellular matrix, extracellular region part, extracellular region, extracellular matrix structural constituent, ECM receptor interaction, collagen-containing extracellular matrix and collagen trimer in the uterus (P < 0.05). Protein-protein interaction analysis revealed that FN1, LOX, THBS2, COL1A1, COL1A2, COL5A1, COL5A2, POSTN, MMP13, VANGL2, RAD54B, SPP1, SDC1, BTC, ANGPTL3 might be key candidate genes for uterine functional maintenance in chicken. This study discovered dominant genes and pathways which enhanced our knowledge of chicken uterine functional maintenance.
禽类蛋壳在子宫中形成。子宫功能的变化可能对蛋壳质量有重大影响。为了确定影响鸡子宫功能维持的重要基因，我们选择了三个不同时期（22W、31W 和 51W）的子宫进行 RNA 测序和生物信息学分析。在我们的研究中，在 W31 与 W22 组、W51 与 W31 组和 W51 与 W22 组中分别检测到 520、706 和 736 个差异表达基因（DEGs）。基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析表明，DEGs 在子宫中富集于细胞外基质、细胞外区域部分、细胞外区域、细胞外基质结构成分、ECM 受体相互作用、含胶原的细胞外基质和胶原三聚体（P<0.05）。蛋白质-蛋白质相互作用分析表明，FN1、LOX、THBS2、COL1A1、COL1A2、COL5A1、COL5A2、POSTN、MMP13、VANGL2、RAD54B、SPP1、SDC1、BTC、ANGPTL3 可能是鸡子宫功能维持的关键候选基因。本研究发现了优势基因和途径，增强了我们对鸡子宫功能维持的认识。
