College of Animal Science and Technology, Guangxi University, Nanning, 530004, China.
BMC Genomics. 2022 Jul 7;23(1):492. doi: 10.1186/s12864-022-08728-w.
During follicular development, a series of key events such as follicular recruitment and selection are crucially governed by strict complex regulation. However, its molecular mechanisms remain obscure. To identify the dominant genes controlling chicken follicular development, the small white follicle (SWF), the small yellow follicle (SYF), and the large yellow follicle (LYF) in different laying stages (W22, W31, W51) were collected for RNA sequencing and bioinformatics analysis. There were 1866, 1211, and 1515 differentially expressed genes (DEGs) between SWF and SYF in W22, W31, and W51, respectively. 4021, 2295, and 2902 DEGs were respectively identified between SYF and LYF in W22, W31, and W51. 5618, 4016, and 4809 DEGs were respectively identified between SWF and LYF in W22, W31, and W51. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that extracellular matrix, extracellular region, extracellular region part, ECM-receptor interaction, collagen extracellular matrix, and collagen trimer were significantly enriched (P < 0.05). Protein-protein interaction analysis revealed that COL4A2, COL1A2, COL4A1, COL5A2, COL12A1, ELN, ALB, and MMP10 might be key candidate genes for follicular development in chicken. The current study identified dominant genes and pathways contributing to our understanding of chicken follicular development.
在卵泡发育过程中，一系列关键事件，如卵泡募集和选择，受到严格复杂的调控。然而，其分子机制尚不清楚。为了鉴定控制鸡卵泡发育的主要基因，本研究从小白卵泡（SWF）、小黄卵泡（SYF）和大黄卵泡（LYF）中采集了不同产蛋阶段（W22、W31、W51）的样本，进行 RNA 测序和生物信息学分析。SWF 和 SYF 在 W22、W31 和 W51 之间分别有 1866、1211 和 1515 个差异表达基因（DEGs）。SYF 和 LYF 在 W22、W31 和 W51 之间分别有 4021、2295 和 2902 个 DEGs。SWF 和 LYF 在 W22、W31 和 W51 之间分别有 5618、4016 和 4809 个 DEGs。GO 和 KEGG 分析表明，细胞外基质、细胞外区域、细胞外区域部分、ECM-受体相互作用、胶原细胞外基质和胶原三聚体显著富集（P<0.05）。蛋白质-蛋白质相互作用分析表明，COL4A2、COL1A2、COL4A1、COL5A2、COL12A1、ELN、ALB 和 MMP10 可能是鸡卵泡发育的关键候选基因。本研究鉴定了主要基因和途径，有助于我们理解鸡卵泡发育。
