Department of Avian Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury, 10-719 Olsztyn, Poland.
Department of Avian Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury, 10-719 Olsztyn, Poland.
Poult Sci. 2020 Jun;99(6):3009-3014. doi: 10.1016/j.psj.2020.03.005. Epub 2020 Mar 20.
Considering the lack of research on the local immunity of the reproductive tract in other poultry species than chickens, the aim of this study was to determine the contribution of T and B cell subpopulations in different parts of breeder turkey oviduct mucous membrane with the use of flow cytometry. In addition, the study aimed to establish an impact of bird age and different stages of the egg production cycle on the systemic and local oviduct-related immune system structure. Our study results demonstrated a lower percentage of T lymphocytes in 32-wk turkey hens followed by a successively increasing population of these cells up to the 38th week of bird's life. The results of our study have also shown a similar dependency between birds' age and number of B lymphocytes. In addition, we demonstrated a decrease in the number of immune system cells in the oviduct, blood, and spleen of turkey hens in the late and end laying period. The differences reported in the number of lymphocyte subpopulations in the reproductive system of laying turkey hens at various stages of the production cycle may, to some extent, explain the frequency and periods of increased predilection to the incidence of infectious diseases in birds under field conditions.
鉴于除了鸡以外，其他家禽生殖道局部免疫的研究较少，本研究旨在使用流式细胞术来确定 T 细胞和 B 细胞亚群在种鸡输卵管黏膜不同部位的贡献。此外，本研究还旨在研究鸟类年龄和产蛋周期不同阶段对全身和局部输卵管相关免疫系统结构的影响。我们的研究结果表明，32 周龄火鸡母鸡的 T 淋巴细胞比例较低，随后这些细胞的数量逐渐增加，直到鸟类生命的第 38 周。我们的研究结果还表明，B 淋巴细胞的数量与鸟类年龄之间存在类似的依赖性。此外，我们还证明了在产蛋后期和末期，火鸡母鸡输卵管、血液和脾脏中的免疫系统细胞数量减少。在产蛋周期的不同阶段，种鸡生殖道中淋巴细胞亚群数量的差异可能在一定程度上解释了在田间条件下，鸟类传染病易感性增加的频率和时期。
