Seoul Institute, National Forensic Service, Jiyangro 139, Yangchungu, Seoul 08036, South Korea.
J Anal Toxicol. 2021 Feb 6;45(1):99-104. doi: 10.1093/jat/bkaa058.
Selegiline (SE) is a selective, irreversible monoamine oxidase-B inhibitor, used for reducing symptoms in early-stage Parkinson's disease. The metabolites of SE include l-methamphetamine, l-amphetamine and desmethylselegiline (DSE). The stereoisomers of SE metabolites, d-methamphetamine and d-amphetamine are highly addictive psychostimulants and some of the most abused drugs in South Korea. In order to differentiate medical SE users form illicit methamphetamine abusers, it is important to distinguish between the l-isomers and d-isomers in urine samples. A 52-year-old male, seemingly under the influence of intoxication and demonstrating abnormal behavior, was reported to the police. The initial urine test using a methamphetamine detection kit demonstrated a positive result. Given the initial results, the police officer requested a further analysis of the urine sample. The urine sample was screened using headspace-solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS). Both methamphetamine and amphetamine were detected, in addition to SE and DSE. To quantitate methamphetamine and amphetamine by HS-SPME-GC-MS, we performed a standard addition method due to the matrix effect of the case sample. Consistent with previous studies, our results indicated that the ratio of amphetamine to methamphetamine was 0.27, which was in the range of SE ingestion. Furthermore, we confirmed l-methamphetamine and l-amphetamine by chiral derivatization using (R)-(-)-α-methoxy-α-(trifluoromethyl) phenylacetyl chloride.
司来吉兰（SE）是一种选择性、不可逆的单胺氧化酶-B 抑制剂，用于减轻早期帕金森病的症状。SE 的代谢物包括 l-甲基苯丙胺、l-苯丙胺和去甲基司来吉兰（DSE）。SE 代谢物的对映异构体，d-甲基苯丙胺和 d-苯丙胺是高度成瘾的精神兴奋剂，也是韩国滥用最多的药物之一。为了将 SE 的医疗使用者与非法甲基苯丙胺使用者区分开来，重要的是要区分尿液样本中的 l-异构体和 d-异构体。一名 52 岁男性，据报道因醉酒和行为异常而被警方报案。最初使用甲基苯丙胺检测试剂盒进行的尿液测试显示阳性结果。鉴于最初的结果，警察要求进一步分析尿液样本。使用顶空固相微萃取-气相色谱-质谱法（HS-SPME-GC-MS）对尿液样本进行了筛选。除了 SE 和 DSE 之外，还检测到了甲基苯丙胺和苯丙胺。为了通过 HS-SPME-GC-MS 定量测定甲基苯丙胺和苯丙胺，由于案例样本的基质效应，我们采用了标准添加法。与先前的研究一致，我们的结果表明，苯丙胺与甲基苯丙胺的比值为 0.27，这处于 SE 摄入范围内。此外，我们通过使用（R）-（-）-α-甲氧基-α-（三氟甲基）苯乙酰氯进行手性衍生化，证实了 l-甲基苯丙胺和 l-苯丙胺的存在。
