Department of Otolaryngology, Umraniye Research and Education Hospital, University of Health Sciences, Elmalıkent Mahallesi Adem Yavuz Caddesi, Umraniye, 34764, Istanbul, Turkey.
Department of Pathology and Molecular Medicine, Umraniye Research and Education Hospital, University of Health Sciences, Istanbul, Turkey.
Eur Arch Otorhinolaryngol. 2020 Nov;277(11):3103-3109. doi: 10.1007/s00405-020-06091-8. Epub 2020 May 31.
OBJECTIVES/HYPOTHESIS: This study was designed to evaluate the graft healing effect of topical application of platelet-rich plasma (PRP) for laryngotracheal reconstruction (LTR) in a rabbit model.
It is a prospective randomized control animal study.
Sixteen healthy New Zealand White rabbits were assigned to two groups of eight animals each. The control group underwent LTR with anterior auricular cartilage graft. The PRP group underwent the same surgical procedure plus PRP application over the anastomosis and surgical field. Two animals in the PRP group and two animals in control group died due to severe respiratory distress on postoperative days 10, 12, 15, and 18. Six rabbits (n = 3 for control group and n = 3 for PRP group) were sacrificed at 4 weeks, and six rabbits (n = 3 for control group and n = 3 for PRP group) were sacrificed at 8 weeks. Laryngotracheal regions were evaluated histopathologically.
Macroscopically, the average anteroposterior and lateral diameter of the reconstructed region and the degree of lumen patency on postoperative 4th week and 8th week were not statistically different among two groups. There was no significant difference between the groups in terms of any of the microscopic findings when the analysis was made separately. However, analysis of the total number of rabbits has shown that new cartilage formation and angiogenesis were more pronounced in PRP group than control group.
Application of PRP contributed to better healing in airway surgery by promoting a release of growth factors that stimulate new cartilage formation and angiogenesis.
目的/假设：本研究旨在评估局部应用富含血小板血浆（PRP）对兔模型喉气管重建（LTR）的移植物愈合效果。
这是一项前瞻性随机对照动物研究。
将 16 只健康的新西兰白兔随机分为两组，每组 8 只。对照组行前耳软骨移植的 LTR。PRP 组行相同的手术，并在吻合口和手术野上加用 PRP。PRP 组的 2 只和对照组的 2 只动物由于术后第 10、12、15 和 18 天严重呼吸困难而死亡。6 只兔子（对照组 n=3，PRP 组 n=3）在 4 周时处死，6 只兔子（对照组 n=3，PRP 组 n=3）在 8 周时处死。对喉气管区域进行组织病理学评估。
大体上，两组在重建区域的前后径和左右径以及术后第 4 周和第 8 周的管腔通畅程度方面无统计学差异。单独分析时，两组之间在任何微观发现方面均无显著差异。然而，对总兔子数的分析表明，PRP 组的新软骨形成和血管生成比对照组更为明显。
PRP 的应用通过释放刺激新软骨形成和血管生成的生长因子，有助于气道手术的更好愈合。
