Honnavar Prasanna, Dogra Sunil, Handa Sanjeev, Chakrabarti Arunaloke, Rudramurthy Shivaprakash M
Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Department of Dermatology, Venereology and Leprosy, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Indian Dermatol Online J. 2020 Mar 9;11(2):167-170. doi: 10.4103/idoj.IDOJ_142_19. eCollection 2020 Mar-Apr.
Pityriasis versicolor (PV) is the most common chronic superficial infection of the stratum corneum, reported in 40-60% of the tropical population. After the description of the new species, only a few studies have been conducted from India.
Molecular identification, quantification of species implicated with PV and correlation to its clinical presentation.
The subjects include 50 PV patients, who attended the dermatology outpatient department of our hospital and 50 healthy individuals. Same size area of the skin was sampled from lesional and non-lesional sites in the patient group and from forehead, cheek, and chest of healthy individuals. spp. isolated were identified by conventional method and confirmed by ITS2 PCR-RFLP and sequencing of D1/D2 region of 26S rDNA.
Eighty percent of patients presented with hypopigmented lesions and 20% with hyperpigmented lesions. From PV lesions, the most frequently isolated species was (50%), followed by (27.3%), mixture of and (15.9%), (4.5%), and (2.3%). Higher Malassezia density was found in lesional area as compared to non-lesional area of PV patients and in healthy individuals ( 0.0001).
Although was the most prevalent species isolated from both patients and controls, significantly higher isolation of from the lesional area compared to non-lesional area indicates its possible role along with in causing PV.
花斑糠疹（PV）是角质层最常见的慢性浅表感染，在40%-60%的热带人群中都有报告。在新物种被描述之后，印度仅开展了少数研究。
进行分子鉴定、定量分析与花斑糠疹相关的物种，并将其与临床表现相关联。
研究对象包括50名前来我院皮肤科门诊就诊的花斑糠疹患者和50名健康个体。从患者组的皮损部位和非皮损部位以及健康个体的前额、脸颊和胸部采集相同面积的皮肤样本。分离出的菌种通过传统方法进行鉴定，并通过ITS2 PCR-RFLP以及26S rDNA D1/D2区域测序进行确认。
80%的患者表现为色素减退性皮损，20%表现为色素沉着性皮损。从花斑糠疹皮损中，最常分离出的菌种是球形马拉色菌（50%），其次是糠秕马拉色菌（27.3%）、球形马拉色菌和糠秕马拉色菌的混合物（15.9%）、合轴马拉色菌（4.5%）以及钝形马拉色菌（2.3%）。与花斑糠疹患者的非皮损部位以及健康个体相比，皮损部位马拉色菌密度更高（P<0.0001）。
尽管球形马拉色菌是从患者和对照中分离出的最常见菌种，但与非皮损部位相比，皮损部位该菌种的分离率显著更高，这表明它可能与糠秕马拉色菌共同在花斑糠疹的发病中发挥作用。
