Westerdijk Fungal Biodiversity Institute, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands.
Department of Genetics and Biotechnology, Faculty of Biology, National and Kapodistrian University of Athens, Panepistimiopolis, Athens 15701, Greece.
FEMS Yeast Res. 2021 Oct 12;21(7). doi: 10.1093/femsyr/foab051.
Malassezia furfur is a yeast species belonging to Malasseziomycetes, Ustilaginomycotina and Basidiomycota that is found on healthy warm-blooded animal skin, but also involved in various skin disorders like seborrheic dermatitis/dandruff and pityriasis versicolor. Moreover, Malassezia are associated with bloodstream infections, Crohn's disease and pancreatic carcinoma. Recent advances in Malassezia genomics and genetics have focused on the nuclear genome. In this work, we present the M. furfur mitochondrial (mt) genetic heterogenicity with full analysis of 14 novel and six available M. furfur mt genomes. The mitogenome analysis reveals a mt gene content typical for fungi, including identification of variable mt regions suitable for intra-species discrimination. Three of them, namely the trnK-atp6 and cox3-nad3 intergenic regions and intron 2 of the cob gene, were selected for primer design to identify strain differences. Malassezia furfur strains belonging to known genetic variable clusters, based on AFLP and nuclear loci, were assessed for their mt variation using PCR amplification and sequencing. The results suggest that these mt regions are excellent molecular markers for the typing of M. furfur strains and may provide added value to nuclear regions when assessing evolutionary relationships at the intraspecies level.
糠秕马拉色菌是一种属于马拉色菌目、外囊菌目和担子菌门的酵母物种，存在于健康的温血动物皮肤上，但也与脂溢性皮炎/头皮屑和花斑癣等各种皮肤疾病有关。此外，马拉色菌与血液感染、克罗恩病和胰腺癌有关。马拉色菌基因组学和遗传学的最新进展集中在核基因组上。在这项工作中，我们展示了完整分析 14 个新的和 6 个现有的糠秕马拉色菌 mt 基因组后的糠秕马拉色菌线粒体（mt）遗传异质性。线粒体基因组分析揭示了真菌特有的 mt 基因含量，包括鉴定适合种内区分的可变 mt 区域。其中三个，即 trnK-atp6 和 cox3-nad3 基因间隔区以及 cob 基因的内含子 2，被选为引物设计，以识别菌株差异。基于 AFLP 和核基因座的已知遗传可变簇的糠秕马拉色菌菌株，使用 PCR 扩增和测序评估其 mt 变异。结果表明，这些 mt 区域是糠秕马拉色菌菌株分型的优秀分子标记，在评估种内水平的进化关系时，可能为核区域提供附加价值。
