Department of Dermatology, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221 005, India.
Indian J Dermatol Venereol Leprol. 2010 Mar-Apr;76(2):159-64. doi: 10.4103/0378-6323.60566.
In the last 10 years, different studies have shown interesting geographical variations in the prevalence of different Malassezia species in pityriasis versicolor.
Identification of Malassezia species isolated from patients with pityriasis versicolor.
In 100 patients with pityriasis versicolor, Malassezia species were identified by culture in Sabouraud's dextrose agar containing cycloheximide with olive oil overlay and modified Dixon agar and by doing biochemical tests (catalase reaction, assimilation of glycine, and Tween utilisation tests).
In 10 patients, 10% KOH smear was negative, while in 90 patients the smear showed characteristic "spaghetti and meatball" appearance. Of these 90 cases, growth was obtained on modified Dixon's agar in 87 cases. Fifty of the isolates (57.5%) were M. globosa, 15 (17.2%) were M. sympodialis, seven (8.0%) were suspected M. sympodialis, 6 (6.9%) each of the isolates were M. furfur and M. obtusa, and three (3.4%) isolates were M. restricta.
M. globosa was the most common species, followed by M. sympodialis, M. furfur, M. obtusa, and M. restricta.
在过去的 10 年中，不同的研究表明不同马拉色菌物种在花斑癣中的流行存在有趣的地理差异。
鉴定花斑癣患者分离的马拉色菌物种。
在 100 例花斑癣患者中，通过在含有环丝氨酸和橄榄油覆盖的沙氏葡萄糖琼脂以及改良 Dixon 琼脂中的培养，并通过进行生化试验（过氧化氢酶反应、甘氨酸同化和吐温利用试验）来鉴定马拉色菌物种。
在 10 例患者中，10% KOH 涂片为阴性，而在 90 例患者中涂片显示出特征性的“意大利面条和肉丸”外观。在这 90 例中，改良 Dixon 琼脂上获得了 87 例生长。50 株分离株（57.5%）为 M. globosa，15 株（17.2%）为 M. sympodialis，7 株（8.0%）为疑似 M. sympodialis，6 株（6.9%）分别为 M. furfur 和 M. obtusa，3 株（3.4%）为 M. restricta。
M. globosa 是最常见的物种，其次是 M. sympodialis、M. furfur、M. obtusa 和 M. restricta。
