Pérez-García Felipe, Jiménez-Sousa Maria Ángeles, Soria Susana, Jorge-Monjas Pablo, Fernández-Rodríguez Amanda, Gómez-Sánchez Esther, Heredia-Rodríguez María, Gómez-Pesquera Estefanía, Martínez-Paz Pedro, Tamayo Eduardo, Resino Salvador
Unidad de Infección Viral e Inmunidad, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Spain.
Departamento de Anestesiología y Reanimación, Hospital Clínico Universitario, Valladolid, Spain.
Front Med (Lausanne). 2020 May 14;7:186. doi: 10.3389/fmed.2020.00186. eCollection 2020.
Interferon lambda 3 (IFNL3, previously called IL-28B) is a cytokine with effects against viral and bacterial pathogens. We aimed to analyze the rs12980275 SNP in patients who underwent major surgery, in order to establish its relationship with susceptibility to septic shock and septic shock-related death in these patients. We performed a case-control study on 376 patients to establish the association between rs12980275 SNP and the susceptibility to develop septic shock. Besides, we performed a longitudinal study among 172 septic shock patients using survival analysis with one censoring point of 28-days mortality. The rs12980275 polymorphism was genotyped by Agena Bioscience's MassARRAY platform. rs12980275 polymorphism was not associated with higher susceptibility to infection and septic shock development. Regarding survival analysis, the Kaplan-Meier analysis showed that patients with rs12980275 AA genotype had higher survival than patients with GG genotype ( = 0.003). The Cox regression analysis adjusted by the most relevant clinical and epidemiological characteristics showed that the GG genotype (recessive model) and the presence of the G allele (additive model) were associated with higher risk of death [adjusted hazard ratio (aHR) = 2.15, = 0.034; aHR = 1.50, = 0.030, respectively]. In conclusion, rs12980275 polymorphism was associated with septic shock-related death in patients who underwent major surgery. The A allele was linked to protection, and the G allele was associated with an increased risk of death. This is a first preliminary study that suggests for the first time a role of polymorphisms in the prognosis of septic shock.
干扰素λ3（IFNL3，以前称为IL-28B）是一种对病毒和细菌病原体有作用的细胞因子。我们旨在分析接受大手术患者中的rs12980275单核苷酸多态性（SNP），以确定其与这些患者感染性休克易感性及感染性休克相关死亡的关系。我们对376例患者进行了病例对照研究，以确定rs12980275 SNP与发生感染性休克易感性之间的关联。此外，我们对172例感染性休克患者进行了纵向研究，采用生存分析，以28天死亡率作为一个截尾点。rs12980275多态性通过Agena Bioscience公司的MassARRAY平台进行基因分型。rs12980275多态性与更高的感染易感性及感染性休克发生无关。关于生存分析，Kaplan-Meier分析显示，rs12980275 AA基因型患者的生存率高于GG基因型患者（P = 0.003）。经最相关的临床和流行病学特征校正后的Cox回归分析显示，GG基因型（隐性模型）和G等位基因的存在（加性模型）与更高的死亡风险相关[校正风险比（aHR）= 2.15，P = 0.034；aHR = 1.50，P = 0.030，分别]。总之，rs12980275多态性与接受大手术患者的感染性休克相关死亡有关。A等位基因与保护作用相关，而G等位基因与死亡风险增加相关。这是第一项初步研究，首次提示了该多态性在感染性休克预后中的作用。
