Dong Wei-Yue, Li Rong, Wang Ying, Tan Jin, Tang Shu-Hua, Jiang Zi-Tao
College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, China.
School of Food Engineering, Tianjin Tianshi College, Tianjin, China.
J Food Biochem. 2020 Aug;44(8):e13293. doi: 10.1111/jfbc.13293. Epub 2020 Jun 1.
The chemical components of an edible spice, sweet ginger (Alpinia coriandriodora D. Fang) essential oil (AEO) were identified by an ultra-fast gas chromatography electronic nose (E-nose) and the main components were β-pinene (27.9%), 1,8-cineole (17.3%), p-cymene (13.5%), camphene (7.3%), myrcene (5.4%), and pseudolimonene (4.3%). The antioxidant activity of each component was evaluated and the mechanisms of scavenging free radical were studied by E-nose combined with chemical methodology. Decanal and α-copaene in AEO showed strong scavenging activities against DPPH radical, and the scavenging rates were over 85%. Decanal had a strong scavenging activity for ABTS radical and the scavenging rate was more than 60%. Similarly, terpinen-4-ol and eugenol had strong scavenging abilities to OH radical and the scavenging rates were more than 50%. Gas chromatography-mass spectrography results showed that it was feasible to identify the chemical components of essential oil by E-nose, and the similarity reached 88.9%. PRACTICAL APPLICATIONS: Many essential oils have antioxidant activities and can be used in functional foods. In the present work, the antioxidant active components in AEO were identified and screened by ultra-fast GC E-nose. We aimed to target the components with strong antioxidant activity quickly and efficiently through the analysis of the reaction process of DPPH, ABTS and OH radicals with a high scavenging rate in a short time. These results indicate that ultra-fast GC E-nose can be used to screen the antioxidant active components in the essential oil.
采用超快速气相色谱电子鼻(E-nose)对食用香料益智砂仁(Alpinia coriandriodora D. Fang)精油(AEO)的化学成分进行了鉴定,其主要成分为β-蒎烯(27.9%)、1,8-桉叶素(17.3%)、对伞花烃(13.5%)、莰烯(7.3%)、月桂烯(5.4%)和假柠檬烯(4.3%)。对各成分的抗氧化活性进行了评价,并结合化学方法研究了其清除自由基的机制。AEO中的癸醛和α-可巴烯对DPPH自由基具有较强的清除活性,清除率均超过85%。癸醛对ABTS自由基具有较强的清除活性,清除率超过60%。同样,萜品-4-醇和丁香酚对OH自由基具有较强的清除能力,清除率超过50%。气相色谱-质谱分析结果表明,利用E-nose鉴定精油化学成分是可行的,相似度达到88.9%。实际应用:许多精油具有抗氧化活性,可用于功能性食品。在本研究中,通过超快速气相色谱电子鼻对AEO中的抗氧化活性成分进行了鉴定和筛选。我们旨在通过在短时间内分析DPPH、ABTS和OH自由基清除率高的反应过程,快速有效地靶向具有强抗氧化活性的成分。这些结果表明,超快速气相色谱电子鼻可用于筛选精油中的抗氧化活性成分。