Tu Pham Thi Be, Tawata Shinkichi
Department of Bioscience and Biotechnology, The United Graduate School of Agricultural Sciences, Kagoshima University, Korimoto 1-21-24, Kagoshima 890-0065, Japan.
Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Senbaru 1, Nishihara-cho, Okinawa 903-0129, Japan.
Molecules. 2015 Sep 14;20(9):16723-40. doi: 10.3390/molecules200916723.
Here, we investigated the anti-oxidant and anti-aging effects of essential oils (EOs) from the leaves of Alpinia zerumbet (tairin and shima) in vitro and anti-melanogenic effects in B16F10 melanoma cells. The anti-oxidant activities were performed with 2,2-diphenyl-1-picrylhydrazyl (DPPH); 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS); nitric oxide; singlet oxygen; hydroxyl radical scavenging; and xanthine oxidase. The inhibitory activities against collagenase, elastase, hyaluronidase, and tyrosinase were employed for anti-aging. The anti-melanogenic was assessed in B16F10 melanoma cells by melanin synthesis and intracellular tyrosinase inhibitory activity. The volatile chemical composition of the essential oil was analyzed with gas chromatography-mass spectrometry (GC/MS). The EO was a complex mixture mainly consisting of monoterpenes and sesquiterpenes. The results revealed that tairin and shima EOs showed strong anti-oxidant activities against DPPH and nitric oxide, hydroxyl radical scavenging activity, and xanthine oxidase inhibition. Compared to shima EO; tairin EO exhibited strong anti-aging activity by inhibiting collagenase, tyrosinase, hyaluronidase, and elastase (IC50 = 11 ± 0.1; 25 ± 1.2; 83 ± 1.6; and 213 ± 2 μg/mL, respectively). Both EOs inhibited intracellular tyrosinase activity; thus, reducing melanin synthesis. These results suggest that tairin EO has better anti-oxidant/anti-aging activity than shima EO, but both are equally anti-melanogenic.
在此，我们研究了艳山姜（太林和shima）叶片精油（EOs）的体外抗氧化和抗衰老作用以及对B16F10黑色素瘤细胞的抗黑色素生成作用。抗氧化活性通过2,2-二苯基-1-苦基肼（DPPH）、2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）二铵盐（ABTS）、一氧化氮、单线态氧、羟基自由基清除和黄嘌呤氧化酶进行测定。采用对胶原酶、弹性蛋白酶、透明质酸酶和酪氨酸酶的抑制活性来评估抗衰老作用。通过黑色素合成和细胞内酪氨酸酶抑制活性在B16F10黑色素瘤细胞中评估抗黑色素生成作用。利用气相色谱-质谱联用仪（GC/MS）分析精油的挥发性化学成分。该精油是一种主要由单萜和倍半萜组成的复杂混合物。结果表明，太林和shima精油对DPPH和一氧化氮表现出较强的抗氧化活性、羟基自由基清除活性以及黄嘌呤氧化酶抑制活性。与shima精油相比，太林精油通过抑制胶原酶、酪氨酸酶、透明质酸酶和弹性蛋白酶表现出较强的抗衰老活性（IC50分别为11±0.1、25±1.2、83±1.6和213±2μg/mL）。两种精油均抑制细胞内酪氨酸酶活性，从而减少黑色素合成。这些结果表明，太林精油比shima精油具有更好的抗氧化/抗衰老活性，但两者的抗黑色素生成能力相当。
