Nickavar Bahman, Adeli Abrisham, Nickavar Azar
Department of Pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Sciences.
J Oleo Sci. 2014;63(7):741-6. doi: 10.5650/jos.ess13168. Epub 2014 Jun 11.
This study was aimed to analyze and identify the antioxidant constituents of the essential oil of Bunium persicum (Apiaceae) fruit. The essential oil was obtained by hydrodistillation and analyses by GC-FID and GC-MS. The essential oil was tested for antioxidant capacity in DPPH radical scavenging and linoleic acid/β-carotene assays. The TLC-bioautography method based on DPPH radical assay and GC analyses were carried out to characterize the major antioxidant compounds in the essential oil. GC analyses showed the presence of sixteen compounds with p-cymene (31.1%), cuminaldehyde (22.2%), and γ-terpinene (11.4%) as the main components in the essential oil. The oil exhibited good radical scavenging [IC50 (DPPH·) = 4.47 (3.96 - 5.05) mg/mL] and antilipid peroxidation [IC50 (β-carotene bleaching) = 0.22 (0.16 - 0.31) mg/mL] activities. The TLC tests resulted in identification of cuminaldehyde, p-cymene-7-ol, and cuminyl acetate as the main constituents of the active oil fraction.
本研究旨在分析和鉴定波斯孜然（伞形科）果实精油中的抗氧化成分。通过水蒸馏法获得精油，并采用气相色谱 - 火焰离子化检测器（GC - FID）和气相色谱 - 质谱联用仪（GC - MS）进行分析。在二苯基苦味酰基自由基（DPPH）清除实验和亚油酸/β - 胡萝卜素实验中对精油的抗氧化能力进行测试。基于DPPH自由基实验的薄层色谱 - 生物自显影法和气相色谱分析用于表征精油中的主要抗氧化化合物。气相色谱分析表明精油中存在16种化合物，其中对伞花烃（31.1%）、枯茗醛（22.2%）和γ - 萜品烯（11.4%）为主要成分。该精油表现出良好的自由基清除活性[IC50（DPPH·） = 4.47（3.96 - 5.05）mg/mL]和抗脂质过氧化活性[IC50（β - 胡萝卜素漂白） = 0.22（0.16 - 0.31）mg/mL]。薄层色谱测试鉴定出枯茗醛、对伞花烃 - 7 - 醇和枯茗醇乙酸酯为活性油馏分的主要成分。
