在细胞 Gd 基定点自旋标记和 eGFP 的 EPR 光谱学中。
In cell Gd-based site-directed spin labeling and EPR spectroscopy of eGFP.
机构信息
Department of Physics, Osnabrück University, Barbarastr. 7, Osnabrück, Germany.
Department of Biology, Osnabrück University, Barbarastr. 11, Osnabrück, Germany.
出版信息
Phys Chem Chem Phys. 2020 Jun 24;22(24):13358-13362. doi: 10.1039/d0cp01930e.
PMID:32478770
Abstract
Label-based functional studies of biomolecules in their native environment require labeling reactions inside living cells. In cell spin labeling using alkyne-azide click chemistry with a Gd3+-DOTAM-azide complex is shown to provide high spin label stability and narrow EPR lines for EPR spectroscopic detection of a spin labeled protein in living cells at ambient temperatures.
摘要
基于标签的生物分子在其天然环境中的功能研究需要在活细胞内进行标记反应。本文展示了在细胞自旋标记中使用炔基-叠氮点击化学反应与 Gd3+-DOTAM-叠氮复合物，为在环境温度下活细胞中自旋标记蛋白的 EPR 光谱检测提供了高自旋标记稳定性和窄 EPR 线。
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